TY - JOUR
T1 - Microarray Analysis of Gene Expression Changes in Neuroplastin 65-Knockout Mice
T2 - Implications for Abnormal Cognition and Emotional Disorders
AU - Li, Huanhuan
AU - Zeng, Jiujiang
AU - Huang, Liang
AU - Wu, Dandan
AU - Liu, Lifen
AU - Liu, Yutong
AU - Yuan, Qionglan
N1 - Funding Information:
Acknowledgements This work was supported by the National Natural Science Foundation of China (81371213, 81070987, and 30971531) and the grants from the Ministry of Science and Technology of China (2010CB945600 and 2010CB945601).
Publisher Copyright:
© 2018, The Author(s).
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Neuroplastin 65 (Np65) is an immunoglobulin superfamily cell adhesion molecule involved in synaptic formation and plasticity. Our recent study showed that Np65-knockout (KO) mice exhibit abnormal cognition and emotional disorders. However, the underlying mechanisms remain unclear. In this study, we found 588 differentially-expressed genes in Np65-KO mice by microarray analysis. RT-PCR analysis also revealed the altered expression of genes associated with development and synaptic structure, such as Cdh1, Htr3a, and Kcnj9. In addition, the expression of Wnt-3, a Wnt protein involved in development, was decreased in Np65-KO mice as evidenced by western blotting. Surprisingly, MRI and DAPI staining showed a significant reduction in the lateral ventricular volume of Np65-KO mice. Together, these findings suggest that ablation of Np65 influences gene expression, which may contribute to abnormal brain development. These results provide clues to the mechanisms underlying the altered brain functions of Np65-deficient mice.
AB - Neuroplastin 65 (Np65) is an immunoglobulin superfamily cell adhesion molecule involved in synaptic formation and plasticity. Our recent study showed that Np65-knockout (KO) mice exhibit abnormal cognition and emotional disorders. However, the underlying mechanisms remain unclear. In this study, we found 588 differentially-expressed genes in Np65-KO mice by microarray analysis. RT-PCR analysis also revealed the altered expression of genes associated with development and synaptic structure, such as Cdh1, Htr3a, and Kcnj9. In addition, the expression of Wnt-3, a Wnt protein involved in development, was decreased in Np65-KO mice as evidenced by western blotting. Surprisingly, MRI and DAPI staining showed a significant reduction in the lateral ventricular volume of Np65-KO mice. Together, these findings suggest that ablation of Np65 influences gene expression, which may contribute to abnormal brain development. These results provide clues to the mechanisms underlying the altered brain functions of Np65-deficient mice.
KW - Gene expression profile
KW - Htr3a
KW - Microarray analysis
KW - Neuroplastin 65
KW - Wnt
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U2 - 10.1007/s12264-018-0251-5
DO - 10.1007/s12264-018-0251-5
M3 - Article
C2 - 29974341
AN - SCOPUS:85049555461
SN - 1673-7067
VL - 34
SP - 779
EP - 788
JO - Neuroscience Bulletin
JF - Neuroscience Bulletin
IS - 5
ER -