Microarray profiling of skeletal muscle sarcoplasmic reticulum proteins

Joseph S. Schulz; Nathan Palmer; Jon Steckelberg; Steven J. Jones; Michael G. Zeece

doi:10.1016/j.bbapap.2006.06.010

Microarray profiling of skeletal muscle sarcoplasmic reticulum proteins

Joseph S. Schulz, Nathan Palmer, Jon Steckelberg, Steven J. Jones, Michael G. Zeece

Food Science and Technology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Microarrays were developed to profile the level of proteins associated with calcium regulation in sarcoplasmic reticulum (SR) isolated from porcine Longissimus muscle. The microarrays consisted of SR preparations printed onto to glass slides and probed with monoclonal antibodies to 7 target proteins. Proteins investigated included: ryanodine receptor, (RyR), dihydropyridine receptor, (DHPR), triadin (TRI), calsequestrin (CSQ), 90 kDa junctional protein (JSR90), and fast-twitch and slow-twitch SR calcium ATPases (SERCA1 and SERCA2). Signal from a fluorescently-labeled detection antibody was measured and quantitated using a slide reader. The microarray developed was also employed to profile Longissimus muscle SR proteins from halothane genotyped animals. Significant (P < 0.05) reductions in levels of several proteins were found including: RyR, CSQ, TRI, DHPR and SERCA2 in SR samples from halothane positive animals. The results illustrate the potential of microarrays as a tool for profiling SR proteins and aiding investigations of calcium regulation.

Original language	English (US)
Pages (from-to)	1429-1435
Number of pages	7
Journal	Biochimica et Biophysica Acta - Proteins and Proteomics
Volume	1764
Issue number	9
DOIs	https://doi.org/10.1016/j.bbapap.2006.06.010
State	Published - Sep 2006

Keywords

Calcium regulation
Protein microarray
Protein profiling
Sarcoplasmic reticulum
Skeletal muscle

ASJC Scopus subject areas

Analytical Chemistry
Biophysics
Biochemistry
Molecular Biology

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10.1016/j.bbapap.2006.06.010

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title = "Microarray profiling of skeletal muscle sarcoplasmic reticulum proteins",

abstract = "Microarrays were developed to profile the level of proteins associated with calcium regulation in sarcoplasmic reticulum (SR) isolated from porcine Longissimus muscle. The microarrays consisted of SR preparations printed onto to glass slides and probed with monoclonal antibodies to 7 target proteins. Proteins investigated included: ryanodine receptor, (RyR), dihydropyridine receptor, (DHPR), triadin (TRI), calsequestrin (CSQ), 90 kDa junctional protein (JSR90), and fast-twitch and slow-twitch SR calcium ATPases (SERCA1 and SERCA2). Signal from a fluorescently-labeled detection antibody was measured and quantitated using a slide reader. The microarray developed was also employed to profile Longissimus muscle SR proteins from halothane genotyped animals. Significant (P < 0.05) reductions in levels of several proteins were found including: RyR, CSQ, TRI, DHPR and SERCA2 in SR samples from halothane positive animals. The results illustrate the potential of microarrays as a tool for profiling SR proteins and aiding investigations of calcium regulation.",

keywords = "Calcium regulation, Protein microarray, Protein profiling, Sarcoplasmic reticulum, Skeletal muscle",

author = "Schulz, {Joseph S.} and Nathan Palmer and Jon Steckelberg and Jones, {Steven J.} and Zeece, {Michael G.}",

note = "Funding Information: This work was supported by USDA grant #16-044 and by the University of Nebraska Agricultural Research Division, Innovative Research Grants program (Journal Series Number 15016).",

year = "2006",

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doi = "10.1016/j.bbapap.2006.06.010",

language = "English (US)",

volume = "1764",

pages = "1429--1435",

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AU - Schulz, Joseph S.

AU - Palmer, Nathan

AU - Steckelberg, Jon

AU - Jones, Steven J.

AU - Zeece, Michael G.

N1 - Funding Information: This work was supported by USDA grant #16-044 and by the University of Nebraska Agricultural Research Division, Innovative Research Grants program (Journal Series Number 15016).

PY - 2006/9

Y1 - 2006/9

N2 - Microarrays were developed to profile the level of proteins associated with calcium regulation in sarcoplasmic reticulum (SR) isolated from porcine Longissimus muscle. The microarrays consisted of SR preparations printed onto to glass slides and probed with monoclonal antibodies to 7 target proteins. Proteins investigated included: ryanodine receptor, (RyR), dihydropyridine receptor, (DHPR), triadin (TRI), calsequestrin (CSQ), 90 kDa junctional protein (JSR90), and fast-twitch and slow-twitch SR calcium ATPases (SERCA1 and SERCA2). Signal from a fluorescently-labeled detection antibody was measured and quantitated using a slide reader. The microarray developed was also employed to profile Longissimus muscle SR proteins from halothane genotyped animals. Significant (P < 0.05) reductions in levels of several proteins were found including: RyR, CSQ, TRI, DHPR and SERCA2 in SR samples from halothane positive animals. The results illustrate the potential of microarrays as a tool for profiling SR proteins and aiding investigations of calcium regulation.

AB - Microarrays were developed to profile the level of proteins associated with calcium regulation in sarcoplasmic reticulum (SR) isolated from porcine Longissimus muscle. The microarrays consisted of SR preparations printed onto to glass slides and probed with monoclonal antibodies to 7 target proteins. Proteins investigated included: ryanodine receptor, (RyR), dihydropyridine receptor, (DHPR), triadin (TRI), calsequestrin (CSQ), 90 kDa junctional protein (JSR90), and fast-twitch and slow-twitch SR calcium ATPases (SERCA1 and SERCA2). Signal from a fluorescently-labeled detection antibody was measured and quantitated using a slide reader. The microarray developed was also employed to profile Longissimus muscle SR proteins from halothane genotyped animals. Significant (P < 0.05) reductions in levels of several proteins were found including: RyR, CSQ, TRI, DHPR and SERCA2 in SR samples from halothane positive animals. The results illustrate the potential of microarrays as a tool for profiling SR proteins and aiding investigations of calcium regulation.

KW - Calcium regulation

KW - Protein microarray

KW - Protein profiling

KW - Sarcoplasmic reticulum

KW - Skeletal muscle

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Microarray profiling of skeletal muscle sarcoplasmic reticulum proteins

Abstract

Keywords

ASJC Scopus subject areas

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