TY - JOUR
T1 - Microbial Transformation of A Sulfonamide Antibiotic Under Various Background Nutrient Conditions
AU - Levine, Rachel E.
AU - Zhang, Yuping
AU - Leng, Yifei
AU - Snow, Daniel D.
AU - Cassada, David
AU - Durso, Lisa M.
AU - Li, Xu
N1 - Funding Information:
The works was supported by the National Science Foundation [Grant No. CBET-1351676]. The funding source was not involved in the design and execution of the study. E-supplementary data of this work can be found in online version of the paper
Funding Information:
The works was supported by the National Science Foundation [Grant No. CBET-1351676]. The funding source was not involved in the design and execution of the study. E-supplementary data of this work can be found in online version of the paper
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Certain microbes can biotransform antibiotics. Little is known about these microbes or the biotransformation processes. The objective of this study was to determine the effects of background nutrient conditions on a sulfonamide degrading culture and on its biotransformation of sulfadiazine (SDZ) with respect to transformation kinetics and transformation products. The mixed culture capable of degrading SDZ consisted primarily of three genera, Brevibacterium, Castellaniella and Leucobacter. The maximum biotransformation rate was 4.55 mg L−1 d−1 in the absence of background nutrients. Among the three background nutrient conditions tested, diluted R2A medium lead to the highest maximum SDZ biotransformation rates, followed by humic acid and glucose. 2-aminopyrimidine was the major SDZ biotransformation product under the background nutrient conditions tested, while another previously reported biotransformation product, sulfanilic acid, was further degraded by the mixed culture. The findings from this study can help improve our estimation of the fate of antibiotics in the environment.
AB - Certain microbes can biotransform antibiotics. Little is known about these microbes or the biotransformation processes. The objective of this study was to determine the effects of background nutrient conditions on a sulfonamide degrading culture and on its biotransformation of sulfadiazine (SDZ) with respect to transformation kinetics and transformation products. The mixed culture capable of degrading SDZ consisted primarily of three genera, Brevibacterium, Castellaniella and Leucobacter. The maximum biotransformation rate was 4.55 mg L−1 d−1 in the absence of background nutrients. Among the three background nutrient conditions tested, diluted R2A medium lead to the highest maximum SDZ biotransformation rates, followed by humic acid and glucose. 2-aminopyrimidine was the major SDZ biotransformation product under the background nutrient conditions tested, while another previously reported biotransformation product, sulfanilic acid, was further degraded by the mixed culture. The findings from this study can help improve our estimation of the fate of antibiotics in the environment.
KW - Antibiotic
KW - Background nutrients
KW - Biotransformation products
KW - Microbial biotransformation
KW - Microbial composition
UR - http://www.scopus.com/inward/record.url?scp=85074013503&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85074013503&partnerID=8YFLogxK
U2 - 10.1007/s00128-019-02727-6
DO - 10.1007/s00128-019-02727-6
M3 - Article
C2 - 31602500
AN - SCOPUS:85074013503
SN - 0007-4861
VL - 103
SP - 808
EP - 813
JO - Bulletin of Environmental Contamination and Toxicology
JF - Bulletin of Environmental Contamination and Toxicology
IS - 6
ER -