Micropatterning of 3D hydrogels for multicellular co-culture

Sudong Kim, Hyung Joon Kim, Jae Hwan Byun, Hyun Ryul Ryu, Qing Ping Hu, Yekyoung Cho, Carlos Pablo Huang, Noo Li Jeon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we present a microfluidic device that allows the precise patterning of hydrogels into distinct 3D layers. The device has channels that partitioned by a series of regularly spaced posts. The posts function as capillary burst valve between adjacent channels allowing multiple gel types to be patterned side by side. In addition, gradients of water-soluble molecules were generated across the patterned hydrogel by varying the spacing between posts that controlled the access to sink and source reservoirs. To demonstrate the utility of this device, primary CNS neurons and astrocytes were co-cultured in the spatially patterned 3D hydrogels.

Original languageEnglish (US)
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1841-1843
Number of pages3
ISBN (Print)9780979806421
StatePublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

Keywords

  • 3D Micropatterning
  • 3D multicellular co-culture

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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