MicroRNA-146a modulates human bronchial epithelial cell survival in response to the cytokine-induced apoptosis

Xiangde Liu, Amy Nelson, Xingqi Wang, Nobuhiro Kanaji, Miok Kim, Tadashi Sato, Masanori Nakanishi, Ying Ji Li, Jianhong Sun, Joel Michalski, Amol Patil, Hesham Basma, Stephen I. Rennard

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

MicroRNA plays an important role in cell differentiation, proliferation and cell death. The current study found that miRNA-146a was up-regulated in human bronchial epithelial cells (HBECs) in response to stimulation by TGF-ß1 plus cytomix (a mixture of IL-1ß, IFN-γ and TNF-α). TGF-ß1 plus cytomix (TCM) induced apoptosis in HBECs (3.4 ± 0.6% of control vs 83.1 ± 4.0% of TCM treated cells, p < 0.01), and this was significantly blocked by the miRNA-146a mimic (8.8 ± 1.5%, p < 0.01). In contrast, a miRNA-146a inhibitor had only a modest effect on cell survival but appeared to augment the induction of epithelial-mesenchymal transition (EMT) in response to the cytokines. The MicroRNA-146a mimic appears to modulate HBEC survival through a mechanism of up-regulating Bcl-XL and STAT3 phosphorylation, and by this mechanism it could contribute to tissue repair and remodeling.

Original languageEnglish (US)
Pages (from-to)177-182
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume380
Issue number1
DOIs
StatePublished - Feb 27 2009

Keywords

  • Apoptosis
  • Bronchial epithelial cell
  • Survival
  • miRNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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