Microsatellite analysis of childhood brain tumors

Hendrik Blaeker, B. K.Ahmed Rasheed, Roger E. McLendon, Henry S. Friedman, Surinder K. Batra, Herbert E. Fuchs, Sandra H. Bigner

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Loss of heterozygosity at specific chromosomal locations has been taken as evidence of a tumor suppressor gene located in that area. We performed a genomic allelotyping study on 46 childhood brain tumors of different histopathological types in order to identify and confirm common areas of deletion in different tumor types. Two hundred microsatellite DNA probes equally distributed over the 22 autosomes were applied, covering the genome in steps of approximately 25 cM. Our results confirm frequent loss of heterozygosity of chromosome arms 9q, 10q, 11p, 11q, 16q, and 22q in high- grade gliomas, medulloblastomas, and ependymomas. In addition, we found a new region of loss on chromosome segment 2p21-23 affected predominantly in high- grade gliomas and medulloblastomas.

Original languageEnglish (US)
Pages (from-to)54-63
Number of pages10
JournalGenes Chromosomes and Cancer
Volume15
Issue number1
DOIs
StatePublished - Jan 1996

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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    Blaeker, H., Rasheed, B. K. A., McLendon, R. E., Friedman, H. S., Batra, S. K., Fuchs, H. E., & Bigner, S. H. (1996). Microsatellite analysis of childhood brain tumors. Genes Chromosomes and Cancer, 15(1), 54-63. https://doi.org/10.1002/(SICI)1098-2264(199601)15:1<54::AID-GCC8>3.0.CO;2-3