Microsatellite genotyping to distinguish colonies and intra-species genetic variation in the eastern subterranean termite, Reticulitermes flavipes (Isoptera: Rhinotermitidae)

Timothy J. Husen, Shripar T. Kamble, Julie M. Stone

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Microsatellite markers and fingerprinting techniques have demonstrated great potential for assessing colony affiliation in Reticulitermes species. This study utilized microsatellite allele fingerprints and examined variation at eight microsatellite loci within five different R. flavipes collection sites in each of Lancaster and Madison counties in Nebraska. In the termite populations from Lancaster County, five of the eight microsatellite loci (Rf 1-3, Rf 3-1, Rf 5-10, Rf 6-1, and Rf 24-2) provided informative genotypic variability allowing colony distinction. DNA sequence data at locus Rf 24-2 also provided conclusive evidence that populations sampled were distinct colonies. Five microsatellite loci (Rf 3-1, Rf 5-10, Rf 6-1, Rf 11-1, and Rf 24-2) were also found to be informative in the termite populations from Madison County. Genotypic diversity by microsatellite allele assignment allowed distinctions between the most closely collected as well as distantly collected colonies.

Original languageEnglish (US)
Pages (from-to)819-835
Number of pages17
JournalSociobiology
Volume48
Issue number3
StatePublished - Nov 9 2006

Keywords

  • Genetic variation
  • Microsatellite markers
  • Nebraska
  • Reticulitermes flavipes
  • Rhinotermitidae

ASJC Scopus subject areas

  • Insect Science

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