Microtubular response to colchicine in adult and fetal rat hepatocytes

Stuart S. Kaufman, Penny L. Blain, Jung H.Y. Park, Dean J. Tuma

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

1. 1. Colchicine and related anti-microtubular drugs impair plasma protein secretion from adult rat liver expiants 2-3-fold more than from fetal tissue. 2. 2. Indirect immunofluorescence microscopy of cultured adult and fetal hepatocytes demonstrated that hepatocytes of both ages contain large numbers of densely packed microtubules which are equally disassembled by 10 μM colchicine. 3. 3. Colchicine (10 μM) reduced secretion of [14C]leucine-labelled proteins from cultured adult hepatocytes by about 50% but did not significantly impede fetal secretion. 4. 4. These results confirmed that plasma protein secretion can proceed without an intact microtubular system in fetal hepatocytes.

Original languageEnglish (US)
Pages (from-to)281-290
Number of pages10
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume95
Issue number2
DOIs
StatePublished - 1990

ASJC Scopus subject areas

  • Physiology

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