Microtubular response to colchicine in adult and fetal rat hepatocytes

Stuart S. Kaufman; Penny L. Blain; Jung H.Y. Park; Dean J. Tuma

doi:10.1016/0300-9629(90)90211-A

Microtubular response to colchicine in adult and fetal rat hepatocytes

Stuart S. Kaufman, Penny L. Blain, Jung H.Y. Park, Dean J. Tuma

Gastroenterology and Hepatology (GI)

Research output: Contribution to journal › Article

7 Scopus citations

Abstract

1. 1. Colchicine and related anti-microtubular drugs impair plasma protein secretion from adult rat liver expiants 2-3-fold more than from fetal tissue. 2. 2. Indirect immunofluorescence microscopy of cultured adult and fetal hepatocytes demonstrated that hepatocytes of both ages contain large numbers of densely packed microtubules which are equally disassembled by 10 μM colchicine. 3. 3. Colchicine (10 μM) reduced secretion of [¹⁴C]leucine-labelled proteins from cultured adult hepatocytes by about 50% but did not significantly impede fetal secretion. 4. 4. These results confirmed that plasma protein secretion can proceed without an intact microtubular system in fetal hepatocytes.

Original language	English (US)
Pages (from-to)	281-290
Number of pages	10
Journal	Comparative Biochemistry and Physiology -- Part A: Physiology
Volume	95
Issue number	2
DOIs	https://doi.org/10.1016/0300-9629(90)90211-A
State	Published - 1990

ASJC Scopus subject areas

Physiology

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10.1016/0300-9629(90)90211-A

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Kaufman, S. S., Blain, P. L., Park, J. H. Y., & Tuma, D. J. (1990). Microtubular response to colchicine in adult and fetal rat hepatocytes. Comparative Biochemistry and Physiology -- Part A: Physiology, 95(2), 281-290. https://doi.org/10.1016/0300-9629(90)90211-A

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AB - 1. 1. Colchicine and related anti-microtubular drugs impair plasma protein secretion from adult rat liver expiants 2-3-fold more than from fetal tissue. 2. 2. Indirect immunofluorescence microscopy of cultured adult and fetal hepatocytes demonstrated that hepatocytes of both ages contain large numbers of densely packed microtubules which are equally disassembled by 10 μM colchicine. 3. 3. Colchicine (10 μM) reduced secretion of [14C]leucine-labelled proteins from cultured adult hepatocytes by about 50% but did not significantly impede fetal secretion. 4. 4. These results confirmed that plasma protein secretion can proceed without an intact microtubular system in fetal hepatocytes.

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