Statement of Purpose: The issue of bone loss in the oral cavity continues to be a perennial problem, especially in the older populations. With the high incidence of dental implants/ fixtures for missing teeth, the success of the surgical procedures in restorative dentistry is dictated by the quality and quantity of the supporting alveolar bone. In order to address the problem of alveolar bone loss and defects due to tumor, periodontitis or even post-extraction remodeling, the present study is the first report on the application of mineralized nanofiber fragments coupled with calcium binding osteoinductive BMP-2 peptides as synthetic graft material for oral bone regeneration. The ease of fabrication and application of cryocut mineralized nanofiber fragments as maxillofacial bone defect fillers present a promising alternative to the current dental bone graft formulations. Furthermore, the nanofiber segments may be utilized for other biomedical applications including soft tissue engineering and wound healing.