MiRNA profiling in pancreatic cancer and restoration of chemosensitivity

Saurabh Singh, Deepak Chitkara, Virender Kumar, Stephen W. Behrman, Ram I. Mahato

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Pancreatic cancers relapse due to small but distinct population of cancer stem cells (CSCs) which are in turn regulated by miRNAs. The present study identifies a series of miRNAs which were either upregulated (e.g. miR-146) or downregulated (e.g. miRNA-205, miRNA-7) in gemcitabine resistant MIA PaCa-2 cancer cells and clinical metastatic pancreatic cancer tissues. Gemcitabine resistant MIA PaCa-2 cells possessed distinct ALDH-positive CSC fraction expressing stem cell markers OCT3/4 and CD44 and chemoresistance marker class III. β-tubulin (TUBB3) which decreases on transfection with miR-205 resulting in the restoration of chemosensitivity to gemcitabine.

Original languageEnglish (US)
Pages (from-to)211-220
Number of pages10
JournalCancer Letters
Volume334
Issue number2
DOIs
StatePublished - Jul 1 2013

Keywords

  • Cancer stem cells
  • Chemoresistance
  • Gemcitabine
  • MiRNA
  • Pancreatic cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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