MiRNA profiling in pancreatic cancer and restoration of chemosensitivity

Saurabh Singh; Deepak Chitkara; Virender Kumar; Stephen W. Behrman; Ram I. Mahato

doi:10.1016/j.canlet.2012.10.008

MiRNA profiling in pancreatic cancer and restoration of chemosensitivity

Saurabh Singh, Deepak Chitkara, Virender Kumar, Stephen W. Behrman, Ram I. Mahato

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Pancreatic cancers relapse due to small but distinct population of cancer stem cells (CSCs) which are in turn regulated by miRNAs. The present study identifies a series of miRNAs which were either upregulated (e.g. miR-146) or downregulated (e.g. miRNA-205, miRNA-7) in gemcitabine resistant MIA PaCa-2 cancer cells and clinical metastatic pancreatic cancer tissues. Gemcitabine resistant MIA PaCa-2 cells possessed distinct ALDH-positive CSC fraction expressing stem cell markers OCT3/4 and CD44 and chemoresistance marker class III. β-tubulin (TUBB3) which decreases on transfection with miR-205 resulting in the restoration of chemosensitivity to gemcitabine.

Original language	English (US)
Pages (from-to)	211-220
Number of pages	10
Journal	Cancer Letters
Volume	334
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2012.10.008
State	Published - Jul 1 2013
Externally published	Yes

Keywords

Cancer stem cells
Chemoresistance
Gemcitabine
MiRNA
Pancreatic cancer

ASJC Scopus subject areas

Oncology
Cancer Research

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title = "MiRNA profiling in pancreatic cancer and restoration of chemosensitivity",

abstract = "Pancreatic cancers relapse due to small but distinct population of cancer stem cells (CSCs) which are in turn regulated by miRNAs. The present study identifies a series of miRNAs which were either upregulated (e.g. miR-146) or downregulated (e.g. miRNA-205, miRNA-7) in gemcitabine resistant MIA PaCa-2 cancer cells and clinical metastatic pancreatic cancer tissues. Gemcitabine resistant MIA PaCa-2 cells possessed distinct ALDH-positive CSC fraction expressing stem cell markers OCT3/4 and CD44 and chemoresistance marker class III. β-tubulin (TUBB3) which decreases on transfection with miR-205 resulting in the restoration of chemosensitivity to gemcitabine.",

keywords = "Cancer stem cells, Chemoresistance, Gemcitabine, MiRNA, Pancreatic cancer",

author = "Saurabh Singh and Deepak Chitkara and Virender Kumar and Behrman, {Stephen W.} and Mahato, {Ram I.}",

note = "Funding Information: The authors would like to thank the Kosten Foundation for financial support.",

year = "2013",

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doi = "10.1016/j.canlet.2012.10.008",

language = "English (US)",

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number = "2",

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TY - JOUR

T1 - MiRNA profiling in pancreatic cancer and restoration of chemosensitivity

AU - Singh, Saurabh

AU - Chitkara, Deepak

AU - Kumar, Virender

AU - Behrman, Stephen W.

AU - Mahato, Ram I.

N1 - Funding Information: The authors would like to thank the Kosten Foundation for financial support.

PY - 2013/7/1

Y1 - 2013/7/1

N2 - Pancreatic cancers relapse due to small but distinct population of cancer stem cells (CSCs) which are in turn regulated by miRNAs. The present study identifies a series of miRNAs which were either upregulated (e.g. miR-146) or downregulated (e.g. miRNA-205, miRNA-7) in gemcitabine resistant MIA PaCa-2 cancer cells and clinical metastatic pancreatic cancer tissues. Gemcitabine resistant MIA PaCa-2 cells possessed distinct ALDH-positive CSC fraction expressing stem cell markers OCT3/4 and CD44 and chemoresistance marker class III. β-tubulin (TUBB3) which decreases on transfection with miR-205 resulting in the restoration of chemosensitivity to gemcitabine.

AB - Pancreatic cancers relapse due to small but distinct population of cancer stem cells (CSCs) which are in turn regulated by miRNAs. The present study identifies a series of miRNAs which were either upregulated (e.g. miR-146) or downregulated (e.g. miRNA-205, miRNA-7) in gemcitabine resistant MIA PaCa-2 cancer cells and clinical metastatic pancreatic cancer tissues. Gemcitabine resistant MIA PaCa-2 cells possessed distinct ALDH-positive CSC fraction expressing stem cell markers OCT3/4 and CD44 and chemoresistance marker class III. β-tubulin (TUBB3) which decreases on transfection with miR-205 resulting in the restoration of chemosensitivity to gemcitabine.

KW - Cancer stem cells

KW - Chemoresistance

KW - Gemcitabine

KW - MiRNA

KW - Pancreatic cancer

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MiRNA profiling in pancreatic cancer and restoration of chemosensitivity

Abstract

Keywords

ASJC Scopus subject areas

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