MiRNA profiling in pancreatic cancer and restoration of chemosensitivity

Saurabh Singh; Deepak Chitkara; Virender Kumar; Stephen W. Behrman; Ram I. Mahato

doi:10.1016/j.canlet.2012.10.008

MiRNA profiling in pancreatic cancer and restoration of chemosensitivity

Saurabh Singh, Deepak Chitkara, Virender Kumar, Stephen W. Behrman, Ram I. Mahato

Research output: Contribution to journal › Article

51 Scopus citations

Abstract

Pancreatic cancers relapse due to small but distinct population of cancer stem cells (CSCs) which are in turn regulated by miRNAs. The present study identifies a series of miRNAs which were either upregulated (e.g. miR-146) or downregulated (e.g. miRNA-205, miRNA-7) in gemcitabine resistant MIA PaCa-2 cancer cells and clinical metastatic pancreatic cancer tissues. Gemcitabine resistant MIA PaCa-2 cells possessed distinct ALDH-positive CSC fraction expressing stem cell markers OCT3/4 and CD44 and chemoresistance marker class III. β-tubulin (TUBB3) which decreases on transfection with miR-205 resulting in the restoration of chemosensitivity to gemcitabine.

Original language	English (US)
Pages (from-to)	211-220
Number of pages	10
Journal	Cancer Letters
Volume	334
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2012.10.008
State	Published - Jul 1 2013

Keywords

Cancer stem cells
Chemoresistance
Gemcitabine
MiRNA
Pancreatic cancer

ASJC Scopus subject areas

Oncology
Cancer Research

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Singh, S., Chitkara, D., Kumar, V., Behrman, S. W., & Mahato, R. I. (2013). MiRNA profiling in pancreatic cancer and restoration of chemosensitivity. Cancer Letters, 334(2), 211-220. https://doi.org/10.1016/j.canlet.2012.10.008

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