Abstract
Pancreatic cancers relapse due to small but distinct population of cancer stem cells (CSCs) which are in turn regulated by miRNAs. The present study identifies a series of miRNAs which were either upregulated (e.g. miR-146) or downregulated (e.g. miRNA-205, miRNA-7) in gemcitabine resistant MIA PaCa-2 cancer cells and clinical metastatic pancreatic cancer tissues. Gemcitabine resistant MIA PaCa-2 cells possessed distinct ALDH-positive CSC fraction expressing stem cell markers OCT3/4 and CD44 and chemoresistance marker class III. β-tubulin (TUBB3) which decreases on transfection with miR-205 resulting in the restoration of chemosensitivity to gemcitabine.
Original language | English (US) |
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Pages (from-to) | 211-220 |
Number of pages | 10 |
Journal | Cancer Letters |
Volume | 334 |
Issue number | 2 |
DOIs | |
State | Published - Jul 1 2013 |
Externally published | Yes |
Keywords
- Cancer stem cells
- Chemoresistance
- Gemcitabine
- MiRNA
- Pancreatic cancer
ASJC Scopus subject areas
- Oncology
- Cancer Research