MiRNA profiling in pancreatic cancer and restoration of chemosensitivity

Saurabh Singh, Deepak Chitkara, Virender Kumar, Stephen W. Behrman, Ram I. Mahato

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Pancreatic cancers relapse due to small but distinct population of cancer stem cells (CSCs) which are in turn regulated by miRNAs. The present study identifies a series of miRNAs which were either upregulated (e.g. miR-146) or downregulated (e.g. miRNA-205, miRNA-7) in gemcitabine resistant MIA PaCa-2 cancer cells and clinical metastatic pancreatic cancer tissues. Gemcitabine resistant MIA PaCa-2 cells possessed distinct ALDH-positive CSC fraction expressing stem cell markers OCT3/4 and CD44 and chemoresistance marker class III. β-tubulin (TUBB3) which decreases on transfection with miR-205 resulting in the restoration of chemosensitivity to gemcitabine.

Original languageEnglish (US)
Pages (from-to)211-220
Number of pages10
JournalCancer Letters
Volume334
Issue number2
DOIs
StatePublished - Jul 1 2013
Externally publishedYes

Keywords

  • Cancer stem cells
  • Chemoresistance
  • Gemcitabine
  • MiRNA
  • Pancreatic cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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