MMP 9 is expressed in PMNs following ischemia/reperfusion injury

M. L. Lindsey, C. R. Keller, P. Jackson, E. Funk, L. H. Michael, M. L. Entman

Research output: Contribution to journalArticlepeer-review


Ischemia/Reperfusion injury in the myocardium generates an inflammatory response that can potentiate damage while at the same time is necessary for healing. Myocardial tissue infiltration of neutrophils (PMN) is a hallmark of this response. PMNs in the reperfused tissue and those isolated from cardiac-specific lymph were examined immunohistochemically and were found to be positive for matrix metalloproteinase 9 (MMP 9), an enzyme involved in matrix remodeling. Gel zymography on cell free lymph revealed the presence of latent MMP 2 and MMP 9. MMP 2 levels did not change over time while MMP 9 levels increased, peaking at 3-8 h and returning to baseline by 24 h post reperfusion. In contrast, in situ zymography demonstrated a reperfusion-dependent increase in gelatinase activity within 3 h. This rise in MMP 9 was temporally and spatially concordant with the amount of PMN infiltration. We suggest that early reperfusion results in PMN infiltration and degranulation. The resultant activated MMP 9 may be a factor in the improved tissue repair seen with reperfusion.

Original languageEnglish (US)
Pages (from-to)A799
JournalFASEB Journal
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics


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