TY - JOUR
T1 - Modeling dermal granulation tissue with the linear fibroblast-populated collagen matrix
T2 - A comparison with the round matrix model
AU - Eichler, Mark J.
AU - Carlson, Mark A.
N1 - Funding Information:
The authors would like to acknowledge the technical assistance of Chris Hansen and Amy Prall. Supported by a grant from the NIH (K08 GM00703) to MAC. MJE was supported by a Summer Research Stipend from the University of Nebraska College of Medicine. The authors also would like to thank Janice Taylor of the Confocal Laser Scanning Microscope Core Facility at the University of Nebraska Medical Center, which is supported by the Nebraska Research Initiative, for providing assistance with confocal microscopy.
PY - 2006/2
Y1 - 2006/2
N2 - Background: Wound contraction typically is not symmetrical; for example, a square-shaped wound will not yield a square scar. Interestingly, the round fibroblast-populated collagen matrix has been used as a model of wound contraction, even though contraction in this model is mostly symmetrical. Objective: We wanted to compare the round versus linear fibroblast-populated collagen matrix to see which would be a better model of dermal granulation tissue. Methods: Gross and microscopic morphology, contraction kinetics, cytoskeletal architecture, and apoptotic and proliferative indices were compared between the round versus the linear fibroblast-populated collagen matrix. A rat excisional wound model was used as an in vivo standard of healing. Results: The rate of contraction was similar between the two models, although the mode of contraction was grossly asymmetric in the linear while remaining symmetric in the round model. Cellular survival and proliferation were both dependent on matrix attachment in both models; this was analogous to the attachment- dependence of granulation tissue. In the attached (restrained) condition, the level of cellular organization was higher in the linear than in the round matrix; the tissue architecture of the linear matrix, moreover, mimicked that of the excisional wound model. Conclusion: The round versus linear fibroblast-populated collagen matrix displayed a similar proliferative and survival response to matrix attachment. The latter model, however, demonstrated tissue organization with attachment and asymmetrical contraction after detachment analogous to that of the in vivo wound model. The linear fibroblast-populated collagen matrix appears to be the better model of dermal granulation tissue.
AB - Background: Wound contraction typically is not symmetrical; for example, a square-shaped wound will not yield a square scar. Interestingly, the round fibroblast-populated collagen matrix has been used as a model of wound contraction, even though contraction in this model is mostly symmetrical. Objective: We wanted to compare the round versus linear fibroblast-populated collagen matrix to see which would be a better model of dermal granulation tissue. Methods: Gross and microscopic morphology, contraction kinetics, cytoskeletal architecture, and apoptotic and proliferative indices were compared between the round versus the linear fibroblast-populated collagen matrix. A rat excisional wound model was used as an in vivo standard of healing. Results: The rate of contraction was similar between the two models, although the mode of contraction was grossly asymmetric in the linear while remaining symmetric in the round model. Cellular survival and proliferation were both dependent on matrix attachment in both models; this was analogous to the attachment- dependence of granulation tissue. In the attached (restrained) condition, the level of cellular organization was higher in the linear than in the round matrix; the tissue architecture of the linear matrix, moreover, mimicked that of the excisional wound model. Conclusion: The round versus linear fibroblast-populated collagen matrix displayed a similar proliferative and survival response to matrix attachment. The latter model, however, demonstrated tissue organization with attachment and asymmetrical contraction after detachment analogous to that of the in vivo wound model. The linear fibroblast-populated collagen matrix appears to be the better model of dermal granulation tissue.
KW - Actin cytoskeleton
KW - Fibroblast-populated collagen matrix
KW - Granulation tissue
KW - Mechanical tension
KW - Wound healing
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U2 - 10.1016/j.jdermsci.2005.09.002
DO - 10.1016/j.jdermsci.2005.09.002
M3 - Article
C2 - 16226016
AN - SCOPUS:30644477692
SN - 0923-1811
VL - 41
SP - 97
EP - 108
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
IS - 2
ER -