TY - CHAP
T1 - Models of metastasis in drug discovery
AU - Talmadge, James E.
PY - 2010
Y1 - 2010
N2 - By definition, animal models provide only an approximation of clinical reality. One reason for this, for example, is that although metastases are the primary cause of mortality from neoplasia, by are rarely considered a target in drug discovery and development. Due to the impact of metastasis on clinical disease, we posit that metastasis should be considered in drug discovery, in addition, to more traditional biologic concepts, including drug pharmacology and toxicity. Drug discovery and developmental studies can incorporate orthotopic and spontaneous metastasis models (syngeneic and xenogeneic) with their inherent host-tumor microenvironmental interactions, in addition to confirmatory autochthonous and/or genetically engineered models (GEMs). This requires a rational and hierarchical approach using models of metastatic disease optimally using resected, orthotopic primary tumors and clinically relevant outcome parameters. In this chapter, we provide protocols for models of metastasis that can be used in translational and drug discovery studies.
AB - By definition, animal models provide only an approximation of clinical reality. One reason for this, for example, is that although metastases are the primary cause of mortality from neoplasia, by are rarely considered a target in drug discovery and development. Due to the impact of metastasis on clinical disease, we posit that metastasis should be considered in drug discovery, in addition, to more traditional biologic concepts, including drug pharmacology and toxicity. Drug discovery and developmental studies can incorporate orthotopic and spontaneous metastasis models (syngeneic and xenogeneic) with their inherent host-tumor microenvironmental interactions, in addition to confirmatory autochthonous and/or genetically engineered models (GEMs). This requires a rational and hierarchical approach using models of metastatic disease optimally using resected, orthotopic primary tumors and clinically relevant outcome parameters. In this chapter, we provide protocols for models of metastasis that can be used in translational and drug discovery studies.
KW - Metastasis
KW - autochthonous tumors
KW - genetically engineered tumor models
KW - orthotopic tumors
KW - spontaneous metastasis
UR - http://www.scopus.com/inward/record.url?scp=77449131744&partnerID=8YFLogxK
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U2 - 10.1007/978-1-60761-058-8_13
DO - 10.1007/978-1-60761-058-8_13
M3 - Chapter
C2 - 20012401
AN - SCOPUS:77449131744
SN - 9781607610571
T3 - Methods in Molecular Biology
SP - 215
EP - 233
BT - Mouse Models for Drug Discovery
A2 - Proetzel, Gabriele
A2 - Wiles, Michael
ER -