Abstract
The secretion of transforming growth factor-α (TGF-α) by several human colon carcinoma cell lines in tissue culture medium was examined. All of the cell lines tested secreted TGF-α to varying degrees. Bio-Gel P-30 chromatography of the conditioned media from three of these cell lines (HCT 116, MOSER, FET) indicated differences in the molecular weights of secreted TGF-α. In the HCT 116 cell line, the majority of the TGF-like activity had a molecular weight of less than 10,000. For both MOSER and FET cell lines, 20-30% of the TGF-like activity had a molecular weight greater than 15,000. When HCT 116 and MOSER cells were treated with differentiation inducing agents there was an increase in a TGF-α species whose molecular weight was greater than 20,000. This indicates a possible alteration in either the processing of the TGF-α precursor and/or secretion of precursor products by the different cell lines.
Original language | English (US) |
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Pages (from-to) | 59-70 |
Number of pages | 12 |
Journal | Cancer Letters |
Volume | 40 |
Issue number | 1 |
DOIs | |
State | Published - May 1988 |
Externally published | Yes |
Keywords
- Colon cancer
- Differentiation
- Transforming growth factor-α
ASJC Scopus subject areas
- Oncology
- Cancer Research