Modulation of a high molecular weight form of transforming growth factor-α in human colon carcinoma cell lines

L. F. Watkins, M. G. Brattain, A. E. Levine

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The secretion of transforming growth factor-α (TGF-α) by several human colon carcinoma cell lines in tissue culture medium was examined. All of the cell lines tested secreted TGF-α to varying degrees. Bio-Gel P-30 chromatography of the conditioned media from three of these cell lines (HCT 116, MOSER, FET) indicated differences in the molecular weights of secreted TGF-α. In the HCT 116 cell line, the majority of the TGF-like activity had a molecular weight of less than 10,000. For both MOSER and FET cell lines, 20-30% of the TGF-like activity had a molecular weight greater than 15,000. When HCT 116 and MOSER cells were treated with differentiation inducing agents there was an increase in a TGF-α species whose molecular weight was greater than 20,000. This indicates a possible alteration in either the processing of the TGF-α precursor and/or secretion of precursor products by the different cell lines.

Original languageEnglish (US)
Pages (from-to)59-70
Number of pages12
JournalCancer Letters
Volume40
Issue number1
DOIs
StatePublished - May 1988
Externally publishedYes

Keywords

  • Colon cancer
  • Differentiation
  • Transforming growth factor-α

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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