Modulation of behavioral networks by selective interneuronal inactivation

M. J. Schmidt, S. Horvath, P. Ebert, J. L. Norris, E. H. Seeley, J. Brown, L. Gellert, M. Everheart, K. A. Garbett, T. W. Grice, R. M. Caprioli, K. Mirnics

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Gamma-aminobutyric acid (GABA)-ergic disturbances are hallmark features of schizophrenia and other neuropsychiatric disorders and encompass multiple interneuronal cell types. Using bacterial artificial chromosome-driven, miRNA silencing technology we generated transgenic mouse lines that suppress glutamic acid decarboxylase 1 (GAD1) in either cholecystokinin (CCK)-or neuropeptide Y (NPY)-expressing interneurons. In situ lipidomic and proteomic analyses on brain tissue sections revealed distinct, brain region-specific profiles in each transgenic line. Behavioral analyses revealed that suppression of GAD1 in CCK+ interneurons resulted in locomotor and olfactory sensory changes, whereas suppression in NPY+ interneurons affected anxiety-related behaviors and social interaction. Both transgenic mouse lines had altered sensitivity to amphetamine albeit in opposite directions. Together, these data argue that reduced GAD1 expression leads to altered molecular and behavioral profiles in a cell type-dependent manner, and that these subpopulations of interneurons are strong and opposing modulators of dopamine system function. Furthermore, our findings also support the hypothesis that neuronal networks are differentially controlled by diverse inhibitory subnetworks.

Original languageEnglish (US)
Pages (from-to)580-587
Number of pages8
JournalMolecular Psychiatry
Issue number5
StatePublished - May 2014
Externally publishedYes


  • GABA
  • GAD1
  • behavior
  • cholecystokinin
  • neuropeptide Y

ASJC Scopus subject areas

  • Molecular Biology
  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience

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