Modulation of network-driven, GABA-mediated giant depolarizing potentials by SDF-1α in the developing hippocampus

Alexander Kasiyanov, Nobutaka Fujii, Hirokazu Tamamura, Huangui Xiong

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Chemokine stromal cell-derived factor-1 (SDF-1, or CXCL12) plays an important role in brain development and functioning. Whole-cell patch clamp recordings were conducted on CA3 neurons in hippocampal slices prepared from neonatal rats between postnatal days 2 and 6 to study the modulatory effects of SDF-1α on network-driven, γ-aminobutyric-acid-mediated giant depolarizing potentials (GDPs), a hallmark of the developing hippocampus. We found that SDF-1α, the only natural ligand for chemokine CXC motif receptor 4 (CXCR4), decreased GDP firing without significant effects on neuronal passive membrane properties in neonatal hippocampal neurons. The SDF-1α-mediated decrease in GDP firing was blocked by T140, a CXCR4 receptor antagonist, suggesting that SDF-1α modulates GDP firing via CXCR4. We also showed that endogenous SDF-1 exerts a tonic inhibitory action on GDPs in the developing hippocampus. As SDF-1/CXCR4 are highly expressed in the developing brain and GDPs are involved in activity-dependent synapse formation and functioning, the inhibitory action of SDF-1α on GDPs may reflect a potential mechanism for chemokine regulation of neural development in early neonatal life.

Original languageEnglish (US)
Pages (from-to)285-292
Number of pages8
JournalDevelopmental Neuroscience
Volume30
Issue number4
DOIs
StatePublished - May 2008

Keywords

  • Chemokine
  • Chemokine CXC motif receptor 4
  • Hippocampal slices
  • Neurodevelopment
  • Whole-cell recordings

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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