Modulation of the urokinase receptor in human colon cell lines by N,N-dimethylformamide

Douglas Boyd, Germaine Florent, Genesio Murano, Michael Brattain

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The present study documents the effect of the planar, polar differentiation promoter N,N-dimethylformamide (DMF) on urokinase binding to colon carcinoma cells. Exposure of the colon carcinoma-cell lines to the agent resulted in enhanced specific binding of radioactive urokinase to all cells tested. Insulin binding to the cells was, however, unaffected by DMF. A DMF exposure period of 45 h was required to observe maximum urokinase binding to two representative cell lines FET and RKO. Optimal stimulation of both cell lines occurred with 0.8% DMF. Scatchard analysis revealed the dissociation constants to be unchanged by the agent with the increased binding of radioactive plasminogen activator reflecting an up-regulation of binding sites. In this regard, the cell line RKO upon exposure to DMF, displayed approx. 700000 receptors/cell, the highest value published, to date, for any cell line.

Original languageEnglish (US)
Pages (from-to)96-100
Number of pages5
JournalBBA - Molecular Cell Research
Volume970
Issue number1
DOIs
StatePublished - Jun 8 1988

Keywords

  • N,N-Dimethylformamide
  • Receptor modulation
  • Urokinase receptor

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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