Molecular Analysis of Peptostreptococcus micros Isolates from Patients with Periodontitis

Craig X. Alpha, Janet M. Guthmiller, Hector E. Cummings, Lori L. Schomberg, Shahriar M. Noorani

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Background: Recent studies provide strong evidence implicating Peptostreptococcus micros in the pathogenesis of various oral infections, including oropharyngeal abscesses and periodontal disease. To date, very little is known regarding the role of P. micros in periodontal disease. Therefore, a genetic analysis was initiated to differentiate among strains of P. micros infecting periodontal patients. Methods: Sixty DNA samples of P. micros isolated from 15 patients with periodontal disease were evaluated. Arbitrarily primed polymerase chain reactions (AP-PCR) were performed using primer 3 (AGTCAGCCAC) and primer 13 (CAGCACC-CAC). The PCR products were analyzed by gel electrophoresis. Results: The primers produced several unique patterns among the strains tested. Primer 3 resulted in 30 different patterns, whereas primer 13 resulted in 31 different patterns, which were distinct from those seen with primer 3. In 8 of 15 patients, the PCR profile was identical for all isolates cultured from that patient, indicating a clonal infection. In 4 of 15 patients, 2 different genotypes were identified. In the remaining 3 patients, all isolates cultured from these patients exhibited a unique genotype. Conclusions: While P. micros appears to be heterogeneous throughout a population of periodontal patients, each patient is, for the most part, infected with a limited number of genotypes. These results demonstrate the genetic diversity of P. micros and the usefulness of AP-PCR for future epidemiological studies in understanding the role P. micros plays in periodontal disease pathogenesis.

Original languageEnglish (US)
Pages (from-to)877-882
Number of pages6
JournalJournal of periodontology
Issue number7
StatePublished - Jul 2001
Externally publishedYes


  • DNA fingerprinting
  • Peptostreptococcus micros
  • Periodontal diseases/microbiology
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Periodontics


Dive into the research topics of 'Molecular Analysis of Peptostreptococcus micros Isolates from Patients with Periodontitis'. Together they form a unique fingerprint.

Cite this