Molecular analysis of the ADH1-Cm allele of maize

John C. Osterman, Elizabeth S. Dennis

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The Adh1-Cmallele and each gene in the Adh1-FCmduplication have been cloned and restriction-mapped. Of the Cmallele 6 kb was sequenced. A single amino acid substitution of aspartate for tyrosine at residue 52 accounts for the altered enzymatic properties of the Cmprotein. Comparison of the nucleotide sequence to that of Adh1-1F and Adh1-1S shows structural and restriction site polymorphisms in the 3′ flanking DNA. Cmlacks the insertion sequence present in 1F and 1S and contains a complex sequence composed of two direct repeats and an inverted repeat. The two genes of the duplication allele have similar restriction maps to Cmand each other.

Original languageEnglish (US)
Pages (from-to)203-212
Number of pages10
JournalPlant Molecular Biology
Volume13
Issue number2
DOIs
StatePublished - Aug 1989

Keywords

  • alcohol dehydrogenase
  • intragenic recombination
  • restriction site polymorphism

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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