Abstract
The Adh1-Cmallele and each gene in the Adh1-FCmduplication have been cloned and restriction-mapped. Of the Cmallele 6 kb was sequenced. A single amino acid substitution of aspartate for tyrosine at residue 52 accounts for the altered enzymatic properties of the Cmprotein. Comparison of the nucleotide sequence to that of Adh1-1F and Adh1-1S shows structural and restriction site polymorphisms in the 3′ flanking DNA. Cmlacks the insertion sequence present in 1F and 1S and contains a complex sequence composed of two direct repeats and an inverted repeat. The two genes of the duplication allele have similar restriction maps to Cmand each other.
Original language | English (US) |
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Pages (from-to) | 203-212 |
Number of pages | 10 |
Journal | Plant Molecular Biology |
Volume | 13 |
Issue number | 2 |
DOIs | |
State | Published - Aug 1989 |
Externally published | Yes |
Keywords
- alcohol dehydrogenase
- intragenic recombination
- restriction site polymorphism
ASJC Scopus subject areas
- Genetics
- Agronomy and Crop Science
- Plant Science