Molecular- and Culture-based Comparison of the Effects of Antimicrobial Agents on Bacterial Survival in Infected Dentinal Tubules

Jerry Cook, Renu Nandakumar, Ashraf F. Fouad

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The aim of this study was to evaluate the effect of root canal obturation with or without prior calcium hydroxide (Ca(OH)2) or 2% chlorhexidine (CHX) on the persistence of bacteria or its DNA in infected dentinal tubules. Canals of 85 extracted teeth were instrumented and inoculated with 104 cells/mL of Enterococcus faecalis. Teeth were incubated at 37°C for 21 days and divided into 3 groups of 25 teeth plus controls. Teeth in group 1 were obturated immediately with gutta-percha (GP) and AH-Plus (Maillefer, Dentsply, Tulsa, OK). In group 2, Ca(OH)2 was placed for 7 days before obturation. In group 3, 10 minutes of irrigation was performed with CHX performed before obturation. After incubation, GP was removed, and dentin specimens were collected and analyzed with culturing and polymerase chain reaction (PCR). No growth occurred in any cultures. By using PCR, E faecalis was detected in fewer roots in group 3 than in groups 1 or 2 (χ2, p = 0.05); 2% CHX treatment followed by obturation was more effective in removing E faecalis DNA than placement of Ca(OH)2 or immediate obturation.

Original languageEnglish (US)
Pages (from-to)690-692
Number of pages3
JournalJournal of endodontics
Volume33
Issue number6
DOIs
StatePublished - Jun 2007

Keywords

  • Calcium hydroxide
  • Enterococcus faecalis
  • chlorhexidine
  • endodontics
  • polymerase chain reaction

ASJC Scopus subject areas

  • Dentistry(all)

Fingerprint Dive into the research topics of 'Molecular- and Culture-based Comparison of the Effects of Antimicrobial Agents on Bacterial Survival in Infected Dentinal Tubules'. Together they form a unique fingerprint.

Cite this