TY - JOUR
T1 - Molecular- and Culture-based Comparison of the Effects of Antimicrobial Agents on Bacterial Survival in Infected Dentinal Tubules
AU - Cook, Jerry
AU - Nandakumar, Renu
AU - Fouad, Ashraf F.
PY - 2007/6
Y1 - 2007/6
N2 - The aim of this study was to evaluate the effect of root canal obturation with or without prior calcium hydroxide (Ca(OH)2) or 2% chlorhexidine (CHX) on the persistence of bacteria or its DNA in infected dentinal tubules. Canals of 85 extracted teeth were instrumented and inoculated with 104 cells/mL of Enterococcus faecalis. Teeth were incubated at 37°C for 21 days and divided into 3 groups of 25 teeth plus controls. Teeth in group 1 were obturated immediately with gutta-percha (GP) and AH-Plus (Maillefer, Dentsply, Tulsa, OK). In group 2, Ca(OH)2 was placed for 7 days before obturation. In group 3, 10 minutes of irrigation was performed with CHX performed before obturation. After incubation, GP was removed, and dentin specimens were collected and analyzed with culturing and polymerase chain reaction (PCR). No growth occurred in any cultures. By using PCR, E faecalis was detected in fewer roots in group 3 than in groups 1 or 2 (χ2, p = 0.05); 2% CHX treatment followed by obturation was more effective in removing E faecalis DNA than placement of Ca(OH)2 or immediate obturation.
AB - The aim of this study was to evaluate the effect of root canal obturation with or without prior calcium hydroxide (Ca(OH)2) or 2% chlorhexidine (CHX) on the persistence of bacteria or its DNA in infected dentinal tubules. Canals of 85 extracted teeth were instrumented and inoculated with 104 cells/mL of Enterococcus faecalis. Teeth were incubated at 37°C for 21 days and divided into 3 groups of 25 teeth plus controls. Teeth in group 1 were obturated immediately with gutta-percha (GP) and AH-Plus (Maillefer, Dentsply, Tulsa, OK). In group 2, Ca(OH)2 was placed for 7 days before obturation. In group 3, 10 minutes of irrigation was performed with CHX performed before obturation. After incubation, GP was removed, and dentin specimens were collected and analyzed with culturing and polymerase chain reaction (PCR). No growth occurred in any cultures. By using PCR, E faecalis was detected in fewer roots in group 3 than in groups 1 or 2 (χ2, p = 0.05); 2% CHX treatment followed by obturation was more effective in removing E faecalis DNA than placement of Ca(OH)2 or immediate obturation.
KW - Calcium hydroxide
KW - Enterococcus faecalis
KW - chlorhexidine
KW - endodontics
KW - polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=34248225264&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34248225264&partnerID=8YFLogxK
U2 - 10.1016/j.joen.2007.01.022
DO - 10.1016/j.joen.2007.01.022
M3 - Article
C2 - 17509407
AN - SCOPUS:34248225264
VL - 33
SP - 690
EP - 692
JO - Journal of Endodontics
JF - Journal of Endodontics
SN - 0099-2399
IS - 6
ER -