Molecular and physiological evaluation of pancreatic cancer-induced cachexia

Surendra K. Shukla, Aneesha Dasgupta, Scott E. Mulder, Pankaj K. Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Cachexia, a complex metabolic syndrome, is characterized by involuntary weight loss along with muscle wasting and fat depletion leading to poor quality of life of patients. About 80% of pancreatic cancer patients exhibit cachectic phenotype at the time of diagnosis. Here, we present the several molecular and physiological parameters, which we utilize to study the pancreatic cancer-induced cachexia in in vitro models and preclinical mice models of pancreatic cancer. We have described myotube and adipocyte-based in vitro models of muscle and fat wasting, including methods of cell culture, differentiation, and treatment with cancer cell-conditioned medium. Furthermore, we have explained the methods of evaluation of key cachectic markers for muscles. Next, we have detailed the orthotopic implantation mouse models of pancreatic cancer and evaluation of different physiological parameters, including body weight, food intake, body composition analysis, glucose tolerance test, insulin resistance test, grip strength measurement, and rotarod performance test. We have also explained morphological parameters and molecular markers to evaluate the muscle wasting in pancreatic cancer-induced cachexia.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages321-333
Number of pages13
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1882
ISSN (Print)1064-3745

Keywords

  • 3T3L1
  • C2C12
  • Cachexia
  • Fat depletion
  • Muscle wasting
  • Orthotopic implantation model
  • Pancreatic cancer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Shukla, S. K., Dasgupta, A., Mulder, S. E., & Singh, P. K. (2019). Molecular and physiological evaluation of pancreatic cancer-induced cachexia. In Methods in Molecular Biology (pp. 321-333). (Methods in Molecular Biology; Vol. 1882). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8879-2_28