Molecular biology III: Amplification of DNA sequences

Research output: Contribution to journalReview articlepeer-review

Abstract

This article discusses some of the tools available to amplify DNA sequences. These techniques are extremely useful for detecting the presence of small amounts of a DNA target sequence. They also can be used to detect short sequences in DNA that has degraded partially. The cloning procedure, useful for producing many copies of a sequence, also is described. Methods for comparing nucleotide sequences within amplified sequences are discussed, including heteroduplex analysis and single-strand conformational polymorphism (SSCP) analysis. Methods for performing DNA sequence analysis are presented as well.

Original languageEnglish (US)
Pages (from-to)191-196
Number of pages6
JournalLaboratory medicine
Volume28
Issue number3
DOIs
StatePublished - Mar 1997

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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