Molecular characterization of a polymerase mutant human immunodeficiency virus

Howard E. Gendelman, Theodore S. Theodore, Ronald Willey, John McCoy, Akio Adachi, Robert J. Mervis, Sundrarajan Venkatesan, Malcolm A. Martin

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


A cell line (8E5) containing a single defective copy of human immunodeficiency virus proviral DNA and producing noninfectious viral particles lacking reverse transcriptase (RT) and endonuclease proteins has recently been described (Folks, et al., (1986b) J. Exp. Med. 164, 280-290). In this report, the mutation in a full-length molecular clone of the provirus (p8E5) was mapped to a 1931-bp region of the pol gene encoding RT. The nucleotide sequence of this segment revealed a 1-base deletion 301 codons from the common amino terminus of the 64- and 51-kDa RTs. Expression of the p8E5 RT segment in Escherichia coli generated an enzymatically inactive and truncated 33-kDa protein.

Original languageEnglish (US)
Pages (from-to)323-329
Number of pages7
Issue number2
StatePublished - Oct 1987
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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