TY - JOUR
T1 - Molecular characterization of methicillin-resistant Staphylococcus aureus strains from pet animals and veterinary staff in China
AU - Zhang, Wanjiang
AU - Hao, Zhihui
AU - Wang, Yang
AU - Cao, Xingyuan
AU - Logue, Catherine M.
AU - Wang, Bing
AU - Yang, Jing
AU - Shen, Jianzhong
AU - Wu, Congming
N1 - Funding Information:
We thank Mei Qiu and Xiu-Mei Wang for their considerable effort in collecting isolates. We also wish to thank Ms. Rachel Sippy (Iowa State University) for revision of the manuscript. This study was supported by grants from the National Natural Science Foundation of China (Nos. 30700604 and 30571401) and the Program for Chang Jiang Scholars and the Innovative Research Team at the University of China (No. IRT0866).
PY - 2011/11
Y1 - 2011/11
N2 - The aim of this study was to determine the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) isolates from pet animals and veterinary staff and the characteristics of these isolates. A total of 22 MRSA isolates were isolated from nasal swabs from dogs, cats and veterinary staff in six pet hospitals in six cities, and examined for antimicrobial susceptibility, the presence of resistance genes, Panton-Valentine leukocidin gene lukF-lukS, staphylococcal chromosomal cassette (SCC) mec typing, spa tying, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. Of 22 MRSA isolates, 21 were recovered from pet animals, and one was isolated from a member of sstaff. All 22 MRSA strains were resistant to penicillin, oxacillin, azithromycin, clindamycin and ceftriaxone, and harboured mecA, ermB and linA genes. The lukF-lukS gene was not detected in any of the MRSA isolates. Eighteen MRSA strains from Qingdao belonged to ST59-MRSA-IV-spa t437. Of four MRSA isolates from Beijing, one belonged to ST398-MRSA-V-spa t034, and three belonged to ST239-MRSA-III-spa t030 profiles. Two PFGE types (A and B) were identified. Two isolates originating from dogs and one isolate originating from a staff member in Beijing shared similar PFGE patterns. Our cumulative data suggested that cross-transmission of MRSA may have occurred between pet animals and veterinary staff.
AB - The aim of this study was to determine the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) isolates from pet animals and veterinary staff and the characteristics of these isolates. A total of 22 MRSA isolates were isolated from nasal swabs from dogs, cats and veterinary staff in six pet hospitals in six cities, and examined for antimicrobial susceptibility, the presence of resistance genes, Panton-Valentine leukocidin gene lukF-lukS, staphylococcal chromosomal cassette (SCC) mec typing, spa tying, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. Of 22 MRSA isolates, 21 were recovered from pet animals, and one was isolated from a member of sstaff. All 22 MRSA strains were resistant to penicillin, oxacillin, azithromycin, clindamycin and ceftriaxone, and harboured mecA, ermB and linA genes. The lukF-lukS gene was not detected in any of the MRSA isolates. Eighteen MRSA strains from Qingdao belonged to ST59-MRSA-IV-spa t437. Of four MRSA isolates from Beijing, one belonged to ST398-MRSA-V-spa t034, and three belonged to ST239-MRSA-III-spa t030 profiles. Two PFGE types (A and B) were identified. Two isolates originating from dogs and one isolate originating from a staff member in Beijing shared similar PFGE patterns. Our cumulative data suggested that cross-transmission of MRSA may have occurred between pet animals and veterinary staff.
KW - Antimicrobial resistance
KW - MLST
KW - MRSA
KW - Pet animals
KW - SCCmec
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U2 - 10.1016/j.tvjl.2011.02.006
DO - 10.1016/j.tvjl.2011.02.006
M3 - Article
C2 - 21382731
AN - SCOPUS:82855178866
SN - 1090-0233
VL - 190
SP - e125-e129
JO - Veterinary Journal
JF - Veterinary Journal
IS - 2
ER -