Molecular characterization of regulatory elements controlling expression of the Bacillus subtilis recA+ gene

D. L. Cheo, K. W. Bayles, R. E. Yasbin

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Expression of the Bacillus subtilis recA gene is induced following DNA damage as well as during the development of the competent state. DNA damage-induction of the recA gene occurs by a RecA-dependent mechanism, whereas competence-induction occurs by a RecA-independent mechanism. To examine the molecular mechanisms that control the expression of the recA gene, a deletion analysis of the recA promoter region was performed. A regulatory region that is required for repression of recA expression was identified upstream of the recA pomoter. Deletion of this regulatory region depressed expression and abolished danage-induction of the recA promoter. Within this region are sequences similar to the consensus that has been identified within DNA damage-inducible promoter regions of other B subtilis genes. Another regulatory region was identified that is required for the RecA-independent, competence-specific induction of the recA gene. Deletion of these sequences significantly reduced competence-induction of the recA promoter.

Original languageEnglish (US)
Pages (from-to)755-762
Number of pages8
JournalBiochimie
Volume74
Issue number7-8
DOIs
StatePublished - 1992

Keywords

  • Bacillus subtilis recA
  • DNA damage
  • gene regulation
  • genetic competence

ASJC Scopus subject areas

  • Biochemistry

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