Molecular clones of the mouse t complex derived from microdissected metaphase chromosomes

Dan Röhme, Howard Fox, Bernhard Herrmann, Anna Maria Frischauf, Jan Erik Edström, Paul Mains, Lee M. Silver, Hans Lehrach

Research output: Contribution to journalArticlepeer-review

140 Scopus citations

Abstract

Fragments of the proximal half of mouse chromosome 17 including the t-complex region were microdissected from metaphase spreads. DNA was isolated from a pool of such fragments, and was cloned on microscale. Individual clones were used to probe genomic digests of DNA from a pair of Chinese hamster cell lines with or without mouse chromosome 17, and livers of congenic inbred lines of mice carrying wild-type and/or t-haplotype forms of chromosome 17. The data obtained indicate that 95% of the low copy number microclone inserts recognize DNA sequences present on mouse chromosome 17. It has been possible to use one-third of these clones to identify restriction-fragment-length polymorphisms between wild-type and t-haplotype DNA on a congenic background. These results demonstrate that these clones have been derived from the t-complex or regions closely linked to it. Clones of this type should provide starting points for a molecular analysis of this region of the mouse genome.

Original languageEnglish (US)
Pages (from-to)783-788
Number of pages6
JournalCell
Volume36
Issue number3
DOIs
StatePublished - Mar 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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