Molecular cloning and characterization of a novel cold-regulated gene from Capsella bursa-pastoris

Sixiu Liu, Xinglong Wang, Zhengqiu Fan, Yongzhen Pang, Xiaofen Sun, Xiangrong Wang, Kexuan Tang

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A novel cor gene was cloned from Capsella bursapastoris (designated Cbcor15b) by RACE-PCR. The full-length cDNA of Cbcor15b was 652bp and contained a 417bp open reading frame (ORF) encoding a 139-amino acid hydrophilic protein. Multiple alignments showed that Cbcor15b had high similarity with other cold-regulated genes from Arabidopsis thaliana (cor15b, cor15a), Brassica napus (bn115, bn19 and bn26) and genes encoding late embryogenesis abundant (LEA) proteins. The predicted CbCOR15B protein was found to have a potential chloroplast signal sequence cleavage site, two cAMP- and cGMP-dependent protein kinase (PKA and PKG) phosphorylation sites. Cold acclimation assay showed that Cbcor15b was relevant to cold acclimation. Our study implies that Cbcor15b might have similar functions possessed by other cor genes in increasing plants' freezing tolerance.

Original languageEnglish (US)
Pages (from-to)262-268
Number of pages7
JournalDNA Sequence - Journal of DNA Sequencing and Mapping
Volume15
Issue number4
DOIs
StatePublished - Aug 2004
Externally publishedYes

Keywords

  • Capsella bursa-pastoris
  • LEA
  • RACE
  • cor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology

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