Molecular cloning and characterization of a novel mannose-binding lectin cDNA from Zantedeschia aethiopica

Zhonghai Chen, Yongzhen Pang, Xiaojun Liu, Xinglong Wang, Zhongxiang Deng, Xiaofen Sun, Kexuan Tang

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Using RNA extracted from Zantedeschia aethiopica young leaves and primers designed according to the conservative regions of Araceae lectins, the full-length cDNA of Z. aethiopica agglutinin (ZAA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zaa was 871 bp and contained a 417 bp open reading frame (ORF) encoding a lectin precursor of 138 amino acids. Through comparative analysis of zaa gene and its deduced amino acid sequence with those of other Araceae species, it was found that zaa encoded a precursor lectin with signal peptide. Secondary and three-dimensional structure analyses showed that ZAA had many common characters of mannose-binding lectin superfamily and ZAA was a mannose-binding lectin with three mannose-binding sites. Southern blot analysis of the genomic DNA revealed that zaa belonged to a multi-copy gene family.

Original languageEnglish (US)
Pages (from-to)187-193
Number of pages7
JournalBiocell
Volume29
Issue number2
StatePublished - Aug 2005
Externally publishedYes

Keywords

  • Mannose-binding lectin
  • RACE
  • ZAA
  • Zantedeschia aethiopica

ASJC Scopus subject areas

  • Cell Biology

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