Molecular cloning and characterization of a novel WRKY gene from Brassica chinensis

X. Liu; X. Wang; Y. Pang; J. Liang; S. Liu; X. Sun; K. Tang

doi:10.1134/S0026893306050074

Molecular cloning and characterization of a novel WRKY gene from Brassica chinensis

X. Liu, X. Wang, Y. Pang, J. Liang, S. Liu, X. Sun, K. Tang

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A new WRKY gene was cloned from Brassica chinensis by rapid amplification of cDNA ends (RACE). The full-length cDNA of BcWRKY was 1175 bp long and contained a 924-bp open reading frame (ORF) encoding a putative W-box-binding protein of 308 amino acids. The predicted BcWRKY protein was found to have a potential bipartite nuclear localization sequence (NLS-PB) in its N-terminal region followed by a WRKY DNA-binding domain. Bioinformatic analysis revealed that BcWRKY resembled other WRKY domain-containing proteins from Arabidopsis thaliana (AtWRKY18), tobacco (WIZZ), parsley (PcWRKY4), and wild oat (ABF2). Expression of the BcWRKY gene could be induced by salicylic acid (SA) and influenced by Pseudomonas syringae pv. tomato strain DC3000 infection and wounding treatment. Our study implies that BcWRKY might have similar functions possessed by other WRKY genes, such as inducing the expression of some defense-related genes and increasing plants' disease resistance ability.

Original language	English (US)
Pages (from-to)	732-740
Number of pages	9
Journal	Molecular Biology
Volume	40
Issue number	5
DOIs	https://doi.org/10.1134/S0026893306050074
State	Published - Oct 2006
Externally published	Yes

Keywords

BcWRKY
Brassica chinensis
Rapid amplification of cDNA ends (RACE)
WRKY domain

ASJC Scopus subject areas

Biophysics
Structural Biology

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@article{66a75fc891e148a48295f011fd477385,

title = "Molecular cloning and characterization of a novel WRKY gene from Brassica chinensis",

abstract = "A new WRKY gene was cloned from Brassica chinensis by rapid amplification of cDNA ends (RACE). The full-length cDNA of BcWRKY was 1175 bp long and contained a 924-bp open reading frame (ORF) encoding a putative W-box-binding protein of 308 amino acids. The predicted BcWRKY protein was found to have a potential bipartite nuclear localization sequence (NLS-PB) in its N-terminal region followed by a WRKY DNA-binding domain. Bioinformatic analysis revealed that BcWRKY resembled other WRKY domain-containing proteins from Arabidopsis thaliana (AtWRKY18), tobacco (WIZZ), parsley (PcWRKY4), and wild oat (ABF2). Expression of the BcWRKY gene could be induced by salicylic acid (SA) and influenced by Pseudomonas syringae pv. tomato strain DC3000 infection and wounding treatment. Our study implies that BcWRKY might have similar functions possessed by other WRKY genes, such as inducing the expression of some defense-related genes and increasing plants' disease resistance ability.",

keywords = "BcWRKY, Brassica chinensis, Rapid amplification of cDNA ends (RACE), WRKY domain",

author = "X. Liu and X. Wang and Y. Pang and J. Liang and S. Liu and X. Sun and K. Tang",

note = "Funding Information: ACKNOWLEDGMENTS This work was funded by the China National Transgenic Plant Research and Commercialization Project, China National 863 High-Tech Program, China Ministry of Education, Shanghai Science and Technology Committee, and Shanghai Agriculture Committee.",

year = "2006",

month = oct,

doi = "10.1134/S0026893306050074",

language = "English (US)",

volume = "40",

pages = "732--740",

journal = "Molecular Biology",

issn = "0026-8933",

publisher = "Pleiades Publishing",

number = "5",

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TY - JOUR

T1 - Molecular cloning and characterization of a novel WRKY gene from Brassica chinensis

AU - Liu, X.

AU - Wang, X.

AU - Pang, Y.

AU - Liang, J.

AU - Liu, S.

AU - Sun, X.

AU - Tang, K.

N1 - Funding Information: ACKNOWLEDGMENTS This work was funded by the China National Transgenic Plant Research and Commercialization Project, China National 863 High-Tech Program, China Ministry of Education, Shanghai Science and Technology Committee, and Shanghai Agriculture Committee.

PY - 2006/10

Y1 - 2006/10

N2 - A new WRKY gene was cloned from Brassica chinensis by rapid amplification of cDNA ends (RACE). The full-length cDNA of BcWRKY was 1175 bp long and contained a 924-bp open reading frame (ORF) encoding a putative W-box-binding protein of 308 amino acids. The predicted BcWRKY protein was found to have a potential bipartite nuclear localization sequence (NLS-PB) in its N-terminal region followed by a WRKY DNA-binding domain. Bioinformatic analysis revealed that BcWRKY resembled other WRKY domain-containing proteins from Arabidopsis thaliana (AtWRKY18), tobacco (WIZZ), parsley (PcWRKY4), and wild oat (ABF2). Expression of the BcWRKY gene could be induced by salicylic acid (SA) and influenced by Pseudomonas syringae pv. tomato strain DC3000 infection and wounding treatment. Our study implies that BcWRKY might have similar functions possessed by other WRKY genes, such as inducing the expression of some defense-related genes and increasing plants' disease resistance ability.

AB - A new WRKY gene was cloned from Brassica chinensis by rapid amplification of cDNA ends (RACE). The full-length cDNA of BcWRKY was 1175 bp long and contained a 924-bp open reading frame (ORF) encoding a putative W-box-binding protein of 308 amino acids. The predicted BcWRKY protein was found to have a potential bipartite nuclear localization sequence (NLS-PB) in its N-terminal region followed by a WRKY DNA-binding domain. Bioinformatic analysis revealed that BcWRKY resembled other WRKY domain-containing proteins from Arabidopsis thaliana (AtWRKY18), tobacco (WIZZ), parsley (PcWRKY4), and wild oat (ABF2). Expression of the BcWRKY gene could be induced by salicylic acid (SA) and influenced by Pseudomonas syringae pv. tomato strain DC3000 infection and wounding treatment. Our study implies that BcWRKY might have similar functions possessed by other WRKY genes, such as inducing the expression of some defense-related genes and increasing plants' disease resistance ability.

KW - BcWRKY

KW - Brassica chinensis

KW - Rapid amplification of cDNA ends (RACE)

KW - WRKY domain

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JO - Molecular Biology

JF - Molecular Biology

IS - 5

ER -

Molecular cloning and characterization of a novel WRKY gene from Brassica chinensis

Abstract

Keywords

ASJC Scopus subject areas

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