Molecular cloning and characterization of the gene encoding the adenine methyltransferase M.CviRI from Chlorella virus XZ-6E

Chris Stefan; Yuannan Xia; James L.van Etten

doi:10.1093/nar/19.2.307

Molecular cloning and characterization of the gene encoding the adenine methyltransferase M.CviRI from Chlorella virus XZ-6E

Chris Stefan, Yuannan Xia, James L.van Etten

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The gene encoding the DNA methyttransferase M.CviRI from Chlorella virus XZ-6E was cloned and expressed in Escherichia coli. M.CviRI methylates adenine in TGCA sequences. DNA containing the M.CviRI gene was sequenced and a single open reading frame of 1137 bp was identified which could code for a polypeptide of 379 amino acids with a predicted molecular weight of 42,814. Comparison of the M.CviRI predicted amino acid sequence with another Chlorella virus and 14 bacterial adenine methyltransferases revealed extensive similarity to the other Chlorella virus enzyme.

Original language	English (US)
Pages (from-to)	307-311
Number of pages	5
Journal	Nucleic acids research
Volume	19
Issue number	2
DOIs	https://doi.org/10.1093/nar/19.2.307
State	Published - Jan 25 1991

ASJC Scopus subject areas

Genetics

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title = "Molecular cloning and characterization of the gene encoding the adenine methyltransferase M.CviRI from Chlorella virus XZ-6E",

abstract = "The gene encoding the DNA methyttransferase M.CviRI from Chlorella virus XZ-6E was cloned and expressed in Escherichia coli. M.CviRI methylates adenine in TGCA sequences. DNA containing the M.CviRI gene was sequenced and a single open reading frame of 1137 bp was identified which could code for a polypeptide of 379 amino acids with a predicted molecular weight of 42,814. Comparison of the M.CviRI predicted amino acid sequence with another Chlorella virus and 14 bacterial adenine methyltransferases revealed extensive similarity to the other Chlorella virus enzyme.",

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