Molecular cloning of a novel LOS2 gene from Capsella bursa-pastoris

Si Xiu Liu, Xing Long Wang, Jin Shui Yang, Zheng Qiu Fan, Xiao Fen Sun, Xiang Rong Wang, Ke Xuan Tang

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A new LOS2 gene was cloned from C. bursa-pastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA of C. bursa-pastoris LOS2 gene (designated as Cblos2) was 1 694 bp containing a 1 332 bp open reading frame (ORF) encoding a 444 amino acid protein. The predicted CbLOS2 protein contained enolase-N domain, enolase domain, conserved putative DNA-binding and repression domains like LOS2 from A. thaliana. Bioinformatic analysis indicated that CbLOS2 had similarity with other enolase proteins. Cold acclimation assay revealed that Cblos2 expressed constitutively in C. bursa-pastoris and was involved in the cold acclimation process, implying CbLOS2 was a bi-functional enolase.

Original languageEnglish (US)
Pages (from-to)600-607
Number of pages8
JournalActa Genetica Sinica
Volume32
Issue number6
StatePublished - Jun 2005
Externally publishedYes

Keywords

  • Capsella bursa-pastoris
  • cDNA
  • Enolase
  • RACE

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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