Molecular cloning of the genes encoding the pr55/bβ/δ regulatory subunits for PP-2A and analysis of their functions in regulating development of goldfish, Carassius auratus

Jun Qiong Zhao, Si Si Xie, Wen Bin Liu, Ya Mei Xiao, Xiao Ming Zeng, Mi Deng, Lili Gong, Jin Ping Liu, Pei Chao Chen, Jie Zhou, Xiao Hui Hu, Jia Han Lv, Xiang Qian Yu, Dao Wang, Chi Li, Yun Lei Peng, Gao Peng Liao, Yun Liu, David Wan Cheng Li

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The protein phosphatase-2A (PP-2A), one of the major phosphatases in eukaryotes, is a heterotrimer, consisting of a scaffold A subunit, a catalytic C subunit and a regulatory B subunit. Previous studies have shown that besides regulating specific PP-2A activity, various B subunits encoded by more than 16 different genes, may have other functions. To explore the possible roles of the regulatory subunits of PP-2A in vertebrate development, we have cloned the PR55/B family regulatory subunits: β and δ, analyzed their tissue specific and developmental expression patterns in Goldfish (Carassius auratus). Our results revealed that the full-length cDNA for PR55/Bβ consists of 1940 bp with an open reading frame of 1332 nucleotides coding for a deduced protein of 443 amino acids. The full length PR55/Bδ cDNA is 2163 bp containing an open reading frame of 1347 nucleotides encoding a deduced protein of 448 amino acids. The two isoforms of PR55/B display high levels of sequence identity with their counterparts in other species. The PR55/Bβ mRNA and protein are detected in brain and heart. In contrast, the PR55/Bδ is expressed in all 9 tissues examined at both mRNA and protein levels. During development of goldfish, the mRNAs for PR55/Bβ and PR55/Bδ show distinct patterns. At the protein level, PR55/Bδ is expressed at all developmental stages examined, suggesting its important role in regulating goldfish development. Expression of the PR55/Bδ anti-sense RNA leads to significant downregulation of PR55/Bδ proteins and caused severe abnormality in goldfish trunk and eye development. Together, our results suggested that PR55/Bδ plays an important role in governing normal trunk and eye formation during goldfish development.

Original languageEnglish (US)
Pages (from-to)135-148
Number of pages14
JournalGene Regulation and Systems Biology
Volume2010
Issue number4
DOIs
StatePublished - 2010

Keywords

  • Developmental regulation
  • Eye
  • Gene expression
  • Lens
  • PP-2A
  • PR55/bβ/δ
  • Phosphorylation
  • Protein phosphatase

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Computer Science Applications

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  • Cite this

    Zhao, J. Q., Xie, S. S., Liu, W. B., Xiao, Y. M., Zeng, X. M., Deng, M., Gong, L., Liu, J. P., Chen, P. C., Zhou, J., Hu, X. H., Lv, J. H., Yu, X. Q., Wang, D., Li, C., Peng, Y. L., Liao, G. P., Liu, Y., & Li, D. W. C. (2010). Molecular cloning of the genes encoding the pr55/bβ/δ regulatory subunits for PP-2A and analysis of their functions in regulating development of goldfish, Carassius auratus. Gene Regulation and Systems Biology, 2010(4), 135-148. https://doi.org/10.4137/GRSB.S6065