Molecular differentiation of alfalfa weevil strains (coleoptera: curculionidae)

S. J. Erney, K. P. Pruess, S. D. Danielson, T. O. Powers

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Mitochondrial DNA was amplified and sequenced from eastern, western, and Egyptian strains of alfalfa weevil. Hypera postica (Gyllenhal). Eastern and Egyptian weevils differed at only 2 nucleotide sites in 1,031 base pairs sequenced; western weevils differed by 5% sequence divergence. Three restriction sites were identified which separated eastern and western haplotypes No intrastrain polymorphism was detected in 150 weevils from Nebraska. Collections from Lincoln in eastern Nebraska and Scottsbluff in western Nebraska were fixed for the eastern and western haplotypes, respectively. Eastern and western haplotypes were found together in the same fields in a broad overlap region in central Nebraska.

Original languageEnglish (US)
Pages (from-to)804-811
Number of pages8
JournalAnnals of the Entomological Society of America
Volume89
Issue number6
DOIs
StatePublished - Nov 1996

Keywords

  • Alfalfa weevil strains
  • DNA sequence
  • Hypera postica
  • PCR-RFLP
  • mtDNA

ASJC Scopus subject areas

  • Insect Science

Fingerprint Dive into the research topics of 'Molecular differentiation of alfalfa weevil strains (coleoptera: curculionidae)'. Together they form a unique fingerprint.

Cite this