Molecular requirement for sterols in herpes simplex virus entry and infectivity

George A. Wudiri; Suzanne M. Pritchard; Hong Li; Jin Liu; Hector C. Aguilar; Stacey D. Gilk; Anthony V. Nicola

doi:10.1128/JVI.01615-14

Molecular requirement for sterols in herpes simplex virus entry and infectivity

George A. Wudiri, Suzanne M. Pritchard, Hong Li, Jin Liu, Hector C. Aguilar, Stacey D. Gilk, Anthony V. Nicola

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Herpes simplex virus 1 (HSV-1) required cholesterol or desmosterol for virion-induced membrane fusion. HSV successfully entered DHCR24^-/- cells, which lack a desmosterol-to-cholesterol conversion enzyme, indicating that entry can occur independently of cholesterol. Depletion of desmosterol from these cells resulted in diminished HSV-1 entry, suggesting a general sterol requirement for HSV-1 entry and that desmosterol can operate in virus entry. Cholesterol functioned more effectively than desmosterol, suggesting that the hydrocarbon tail of cholesterol influences viral entry.

Original language	English (US)
Pages (from-to)	13918-13922
Number of pages	5
Journal	Journal of virology
Volume	88
Issue number	23
DOIs	https://doi.org/10.1128/JVI.01615-14
State	Published - 2014
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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title = "Molecular requirement for sterols in herpes simplex virus entry and infectivity",

abstract = "Herpes simplex virus 1 (HSV-1) required cholesterol or desmosterol for virion-induced membrane fusion. HSV successfully entered DHCR24-/- cells, which lack a desmosterol-to-cholesterol conversion enzyme, indicating that entry can occur independently of cholesterol. Depletion of desmosterol from these cells resulted in diminished HSV-1 entry, suggesting a general sterol requirement for HSV-1 entry and that desmosterol can operate in virus entry. Cholesterol functioned more effectively than desmosterol, suggesting that the hydrocarbon tail of cholesterol influences viral entry.",

author = "Wudiri, {George A.} and Pritchard, {Suzanne M.} and Hong Li and Jin Liu and Aguilar, {Hector C.} and Gilk, {Stacey D.} and Nicola, {Anthony V.}",

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AU - Wudiri, George A.

AU - Pritchard, Suzanne M.

AU - Li, Hong

AU - Liu, Jin

AU - Aguilar, Hector C.

AU - Gilk, Stacey D.

AU - Nicola, Anthony V.

PY - 2014

Y1 - 2014

N2 - Herpes simplex virus 1 (HSV-1) required cholesterol or desmosterol for virion-induced membrane fusion. HSV successfully entered DHCR24-/- cells, which lack a desmosterol-to-cholesterol conversion enzyme, indicating that entry can occur independently of cholesterol. Depletion of desmosterol from these cells resulted in diminished HSV-1 entry, suggesting a general sterol requirement for HSV-1 entry and that desmosterol can operate in virus entry. Cholesterol functioned more effectively than desmosterol, suggesting that the hydrocarbon tail of cholesterol influences viral entry.

AB - Herpes simplex virus 1 (HSV-1) required cholesterol or desmosterol for virion-induced membrane fusion. HSV successfully entered DHCR24-/- cells, which lack a desmosterol-to-cholesterol conversion enzyme, indicating that entry can occur independently of cholesterol. Depletion of desmosterol from these cells resulted in diminished HSV-1 entry, suggesting a general sterol requirement for HSV-1 entry and that desmosterol can operate in virus entry. Cholesterol functioned more effectively than desmosterol, suggesting that the hydrocarbon tail of cholesterol influences viral entry.

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Molecular requirement for sterols in herpes simplex virus entry and infectivity

Abstract

ASJC Scopus subject areas

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