Molecular requirement for sterols in herpes simplex virus entry and infectivity

George A. Wudiri, Suzanne M. Pritchard, Hong Li, Jin Liu, Hector C. Aguilar, Stacey D. Gilk, Anthony V. Nicola

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Herpes simplex virus 1 (HSV-1) required cholesterol or desmosterol for virion-induced membrane fusion. HSV successfully entered DHCR24-/- cells, which lack a desmosterol-to-cholesterol conversion enzyme, indicating that entry can occur independently of cholesterol. Depletion of desmosterol from these cells resulted in diminished HSV-1 entry, suggesting a general sterol requirement for HSV-1 entry and that desmosterol can operate in virus entry. Cholesterol functioned more effectively than desmosterol, suggesting that the hydrocarbon tail of cholesterol influences viral entry.

Original languageEnglish (US)
Pages (from-to)13918-13922
Number of pages5
JournalJournal of virology
Volume88
Issue number23
DOIs
StatePublished - 2014
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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