Molecular serotyping of Escherichia coli O111:H8

Lisa M. Durso; James L. Bono; James E. Keen

doi:10.1016/j.mimet.2007.01.016

Molecular serotyping of Escherichia coli O111:H8

Lisa M. Durso, James L. Bono, James E. Keen

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Accurate Escherichia coli serotyping is critical for pathogen diagnosis and surveillance of non-O157 Shiga-toxigenic strains, however few laboratories have this capacity. The molecular serotyping protocol described in this paper targets the somatic and flagellar antigens of E. coli O111:H8 used in traditional serotyping, and can be performed routinely in the laboratory.

Original language	English (US)
Pages (from-to)	381-383
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	69
Issue number	2
DOIs	https://doi.org/10.1016/j.mimet.2007.01.016
State	Published - May 2007
Externally published	Yes

Keywords

E. coli
E. coli O111:H8
Molecular serotyping
O111:H8
Serotyping

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2007.01.016

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title = "Molecular serotyping of Escherichia coli O111:H8",

abstract = "Accurate Escherichia coli serotyping is critical for pathogen diagnosis and surveillance of non-O157 Shiga-toxigenic strains, however few laboratories have this capacity. The molecular serotyping protocol described in this paper targets the somatic and flagellar antigens of E. coli O111:H8 used in traditional serotyping, and can be performed routinely in the laboratory.",

keywords = "E. coli, E. coli O111:H8, Molecular serotyping, O111:H8, Serotyping",

author = "Durso, {Lisa M.} and Bono, {James L.} and Keen, {James E.}",

note = "Funding Information: This work was supported, in part, by USDA CSREES NRI grant #2002-2239 (awarded to JEK) and the USDA-ARS. We thank Ron Mlejnek, Sandy Fryda-Bradley, Liz Ossian, Randy Bradley, Steve Simcox, and Bob Lee for technical assistance, and Joan Rosch for secretarial assistance. Thanks to the Centers for Disease Control and Prevention, Foodborne and Diarrheal Disease Branch for supplying the O antigen standard strains. ",

year = "2007",

month = may,

doi = "10.1016/j.mimet.2007.01.016",

language = "English (US)",

volume = "69",

pages = "381--383",

journal = "Journal of Microbiological Methods",

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publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Molecular serotyping of Escherichia coli O111:H8

AU - Durso, Lisa M.

AU - Bono, James L.

AU - Keen, James E.

N1 - Funding Information: This work was supported, in part, by USDA CSREES NRI grant #2002-2239 (awarded to JEK) and the USDA-ARS. We thank Ron Mlejnek, Sandy Fryda-Bradley, Liz Ossian, Randy Bradley, Steve Simcox, and Bob Lee for technical assistance, and Joan Rosch for secretarial assistance. Thanks to the Centers for Disease Control and Prevention, Foodborne and Diarrheal Disease Branch for supplying the O antigen standard strains.

PY - 2007/5

Y1 - 2007/5

N2 - Accurate Escherichia coli serotyping is critical for pathogen diagnosis and surveillance of non-O157 Shiga-toxigenic strains, however few laboratories have this capacity. The molecular serotyping protocol described in this paper targets the somatic and flagellar antigens of E. coli O111:H8 used in traditional serotyping, and can be performed routinely in the laboratory.

AB - Accurate Escherichia coli serotyping is critical for pathogen diagnosis and surveillance of non-O157 Shiga-toxigenic strains, however few laboratories have this capacity. The molecular serotyping protocol described in this paper targets the somatic and flagellar antigens of E. coli O111:H8 used in traditional serotyping, and can be performed routinely in the laboratory.

KW - E. coli

KW - E. coli O111:H8

KW - Molecular serotyping

KW - O111:H8

KW - Serotyping

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JF - Journal of Microbiological Methods

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ER -

Molecular serotyping of Escherichia coli O111:H8

Abstract

Keywords

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