TY - JOUR
T1 - Molecular structure of the rearranged t-cell gamma chain gene in a human leukemia which expresses its product
AU - González-Sarmiento, Rogelio
AU - Pirruccello, Samuel J.
AU - Wilkowski, Christopher W.
AU - Griesinger, Frank
AU - Greenberg, James M.
AU - Kersey, John H.
N1 - Funding Information:
Supported in part by the following Grants: NIH CA21737 and CA255097, the Leukemia Research Fund. RGS is the recipient of a Fulbright Fellowship awarded by the Spanish Council of Education. JMG is a Special Fellow of the Leukemia Society of America.
PY - 1990
Y1 - 1990
N2 - The gamma gene product is a component of the second T-cell receptor. We report a new case of acute lymphoblastic leukemia bearing a CD3+ CD4- CD5+ CD7+ CD8- WT31- immunophenpotype that expresses the gamma peptide. Immunoprecipitation studies using an anti Cγ heteroantisera showed two different bands of 40 and 60 Kd. Southern analysis revealed Cγ1 utilization in the productive rearrangement. The demonstration of VδJδ1 rearrangement in this leukemia suggests that the 60 Kd band could correspond to the product of the delta gene. The utilization of the Jγ1.3 exon in this leukemia suggests that the T lymphocytes that undergo leukemic transformation are derived from a population different from the circulating γdelta; lymphocytes, that preferentially use the Jγ1.2 (JγP) exon.
AB - The gamma gene product is a component of the second T-cell receptor. We report a new case of acute lymphoblastic leukemia bearing a CD3+ CD4- CD5+ CD7+ CD8- WT31- immunophenpotype that expresses the gamma peptide. Immunoprecipitation studies using an anti Cγ heteroantisera showed two different bands of 40 and 60 Kd. Southern analysis revealed Cγ1 utilization in the productive rearrangement. The demonstration of VδJδ1 rearrangement in this leukemia suggests that the 60 Kd band could correspond to the product of the delta gene. The utilization of the Jγ1.3 exon in this leukemia suggests that the T lymphocytes that undergo leukemic transformation are derived from a population different from the circulating γdelta; lymphocytes, that preferentially use the Jγ1.2 (JγP) exon.
KW - Acute lymphoblastic leukemia
KW - Gamma chain T-cell receptor
KW - Gene rearrangement
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U2 - 10.3109/10428199009050989
DO - 10.3109/10428199009050989
M3 - Article
C2 - 27457301
AN - SCOPUS:0025563270
SN - 1042-8194
VL - 3
SP - 145
EP - 150
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
IS - 2
ER -