Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting.

C. L. Kelling, J. E. Kennedy, K. K. Rump, L. C. Stine, P. S. Paul, J. E. Partridge

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Viral RNA oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (BVDV) grown in medium supplemented with serum contaminated with noncytopathic BVDV from the same 3 viruses grown in cell culture free of BVDV. Oligonucleotide fingerprinting also effectively discriminated between reference Singer BVDV, NADL BVDV, and New York-1 BVDV grown in BVDV-free noncontaminated or BVDV-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral RNA maybe used to determine the purity of virus stocks, as well as that of BVDV vaccines.

Original languageEnglish (US)
Pages (from-to)1237-1244
Number of pages8
JournalAmerican journal of veterinary research
Volume52
Issue number8
StatePublished - Aug 1991

ASJC Scopus subject areas

  • veterinary(all)

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