Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting.

C. L. Kelling; J. E. Kennedy; K. K. Rump; L. C. Stine; P. S. Paul; J. E. Partridge

Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting.

C. L. Kelling, J. E. Kennedy, K. K. Rump, L. C. Stine, P. S. Paul, J. E. Partridge

Research output: Contribution to journal › Article › peer-review

Abstract

Viral RNA oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (BVDV) grown in medium supplemented with serum contaminated with noncytopathic BVDV from the same 3 viruses grown in cell culture free of BVDV. Oligonucleotide fingerprinting also effectively discriminated between reference Singer BVDV, NADL BVDV, and New York-1 BVDV grown in BVDV-free noncontaminated or BVDV-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral RNA maybe used to determine the purity of virus stocks, as well as that of BVDV vaccines.

Original language	English (US)
Pages (from-to)	1237-1244
Number of pages	8
Journal	American journal of veterinary research
Volume	52
Issue number	8
State	Published - Aug 1991

ASJC Scopus subject areas

General Veterinary

Cite this

@article{0891e8949cea416488e0a8603c27c91d,

title = "Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting.",

abstract = "Viral RNA oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (BVDV) grown in medium supplemented with serum contaminated with noncytopathic BVDV from the same 3 viruses grown in cell culture free of BVDV. Oligonucleotide fingerprinting also effectively discriminated between reference Singer BVDV, NADL BVDV, and New York-1 BVDV grown in BVDV-free noncontaminated or BVDV-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral RNA maybe used to determine the purity of virus stocks, as well as that of BVDV vaccines.",

author = "Kelling, {C. L.} and Kennedy, {J. E.} and Rump, {K. K.} and Stine, {L. C.} and Paul, {P. S.} and Partridge, {J. E.}",

year = "1991",

month = aug,

language = "English (US)",

volume = "52",

pages = "1237--1244",

journal = "American journal of veterinary research",

issn = "0002-9645",

publisher = "American Veterinary Medical Association",

number = "8",

}

TY - JOUR

T1 - Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting.

AU - Kelling, C. L.

AU - Kennedy, J. E.

AU - Rump, K. K.

AU - Stine, L. C.

AU - Paul, P. S.

AU - Partridge, J. E.

PY - 1991/8

Y1 - 1991/8

N2 - Viral RNA oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (BVDV) grown in medium supplemented with serum contaminated with noncytopathic BVDV from the same 3 viruses grown in cell culture free of BVDV. Oligonucleotide fingerprinting also effectively discriminated between reference Singer BVDV, NADL BVDV, and New York-1 BVDV grown in BVDV-free noncontaminated or BVDV-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral RNA maybe used to determine the purity of virus stocks, as well as that of BVDV vaccines.

AB - Viral RNA oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (BVDV) grown in medium supplemented with serum contaminated with noncytopathic BVDV from the same 3 viruses grown in cell culture free of BVDV. Oligonucleotide fingerprinting also effectively discriminated between reference Singer BVDV, NADL BVDV, and New York-1 BVDV grown in BVDV-free noncontaminated or BVDV-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral RNA maybe used to determine the purity of virus stocks, as well as that of BVDV vaccines.

UR - http://www.scopus.com/inward/record.url?scp=0026212239&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026212239&partnerID=8YFLogxK

M3 - Article

C2 - 1656821

AN - SCOPUS:0026212239

SN - 0002-9645

VL - 52

SP - 1237

EP - 1244

JO - American journal of veterinary research

JF - American journal of veterinary research

IS - 8

ER -

Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting.

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this