Abstract
Mouse monoclonal antibodies (MAb) have been generated against the anionic isozyme of human glutathione S‐transferase (GST π). MAb AGST I can inhibit 50‐70% of GST π enzymatic activity and reacts with a 3‐dimensional epitope which includes a putative glutathione binding site on GST π. A sandwich enzyme‐immunoassay established using MAb AGST I and a polyclonal antibody displayed a sensitivity of 0.5 ng/ml. Immunohistochemical analysis of human tissues demonstrated marked increases in GST π levels in cancers of the brain, cervix, endometrium, colon, rectum and testis and in fibro‐and chondrosarcomas.
Original language | English (US) |
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Pages (from-to) | 193-201 |
Number of pages | 9 |
Journal | International Journal of Cancer |
Volume | 47 |
Issue number | 2 |
DOIs | |
State | Published - Jan 21 1991 |
Externally published | Yes |
ASJC Scopus subject areas
- Oncology
- Cancer Research