Monoclonal antibodies to glutathione S‐Transferase π–immunohistochemical analysis of human tissues and cancers

Raphe R.S. Kantor, Steven L. Giardina, Armando Bartolazzi, Allen J. Townsend, Charles E. Myers, Kenneth H. Cowan, Dan L. Longo, Pier G. Natali

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Abstract

Mouse monoclonal antibodies (MAb) have been generated against the anionic isozyme of human glutathione S‐transferase (GST π). MAb AGST I can inhibit 50‐70% of GST π enzymatic activity and reacts with a 3‐dimensional epitope which includes a putative glutathione binding site on GST π. A sandwich enzyme‐immunoassay established using MAb AGST I and a polyclonal antibody displayed a sensitivity of 0.5 ng/ml. Immunohistochemical analysis of human tissues demonstrated marked increases in GST π levels in cancers of the brain, cervix, endometrium, colon, rectum and testis and in fibro‐and chondrosarcomas.

Original languageEnglish (US)
Pages (from-to)193-201
Number of pages9
JournalInternational Journal of Cancer
Volume47
Issue number2
DOIs
StatePublished - Jan 21 1991

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Kantor, R. R. S., Giardina, S. L., Bartolazzi, A., Townsend, A. J., Myers, C. E., Cowan, K. H., Longo, D. L., & Natali, P. G. (1991). Monoclonal antibodies to glutathione S‐Transferase π–immunohistochemical analysis of human tissues and cancers. International Journal of Cancer, 47(2), 193-201. https://doi.org/10.1002/ijc.2910470206