Monoclonal Antibody against Mouse CAR following Genetic Immunization

Steven D. Carson, Barbara L. Switzer, Steven M. Tracy, Nora M. Chapman

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

To broaden our repertoire of monoclonal antibodies against CAR (coxsackievirus and adenovirus receptor), we inoculated mice with an expression vector containing the cDNA encoding human CAR extracellular and transmembrane sequence, and boosted the response by inoculation with soluble human CAR protein produced in E. coli. Of the hybridomas obtained following this immunization protocol, one secreted IgG with exceptional reactivity against mouse CAR. Since CAR has been shown to form dimers, expression of human CAR in cells that express mouse CAR may have stimulated the host immune system to recognize endogenous CAR in heterodimers.

Original languageEnglish (US)
Pages (from-to)19-22
Number of pages4
JournalHybridoma and Hybridomics
Volume23
Issue number1
DOIs
StatePublished - Feb 2004

ASJC Scopus subject areas

  • Immunology
  • Genetics

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