TY - JOUR
T1 - Morphology and Proteome Characterization of the Salivary Glands of the Western Chinch Bug (Hemiptera
T2 - Blissidae)
AU - Ramm, Crystal
AU - Wayadande, Astri
AU - Baird, Lisa
AU - Nandakumar, Renu
AU - Madayiputhiya, Nandakumar
AU - Amundsen, Keenan
AU - Donze-Reiner, Teresa
AU - Baxendale, Frederick
AU - Sarath, Gautam
AU - Heng-Moss, Tiffany
N1 - Publisher Copyright:
© The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2015
Y1 - 2015
N2 - The western chinch bug, Blissus occiduus Barber, is a serious pest of buffalograss, Buchloe dactyloides (Nuttall) due to physical and chemical damage caused during the feeding process. Although previous work has investigated the feeding behaviors of chinch bugs in the Blissus complex, no study to date has explored salivary gland morphology and the associated salivary complex of this insect. Whole and sectioned B. occiduus salivary glands were visualized using light and scanning electron microscopy to determine overall structure and cell types of the salivary glands and their individual lobes. Microscopy revealed a pair of trilobed principal glands and a pair of tubular accessory glands of differing cellular types. To link structure with function, the salivary gland proteome was characterized using liquid chromatography tandem mass spectrometry. The salivary proteome analysis resulted in B. occiduus sequences matching 228 nonhomologous protein sequences of the pea aphid, Acyrthosiphon pisum (Harris), with many specific to the proteins present in the salivary proteome of A. pisum. A number of sequences were assigned the molecular function of hydrolase and oxido-reductase activity, with one specific protein sequence revealing a peroxidase-like function. This is the first study to characterize the salivary proteome of B. occiduus and the first of any species in the family Blissidae.
AB - The western chinch bug, Blissus occiduus Barber, is a serious pest of buffalograss, Buchloe dactyloides (Nuttall) due to physical and chemical damage caused during the feeding process. Although previous work has investigated the feeding behaviors of chinch bugs in the Blissus complex, no study to date has explored salivary gland morphology and the associated salivary complex of this insect. Whole and sectioned B. occiduus salivary glands were visualized using light and scanning electron microscopy to determine overall structure and cell types of the salivary glands and their individual lobes. Microscopy revealed a pair of trilobed principal glands and a pair of tubular accessory glands of differing cellular types. To link structure with function, the salivary gland proteome was characterized using liquid chromatography tandem mass spectrometry. The salivary proteome analysis resulted in B. occiduus sequences matching 228 nonhomologous protein sequences of the pea aphid, Acyrthosiphon pisum (Harris), with many specific to the proteins present in the salivary proteome of A. pisum. A number of sequences were assigned the molecular function of hydrolase and oxido-reductase activity, with one specific protein sequence revealing a peroxidase-like function. This is the first study to characterize the salivary proteome of B. occiduus and the first of any species in the family Blissidae.
KW - Hemiptera
KW - chinch bug
KW - salivary gland
KW - salivary proteome
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U2 - 10.1093/jee/tov149
DO - 10.1093/jee/tov149
M3 - Article
C2 - 26470353
AN - SCOPUS:84943801606
VL - 108
SP - 2055
EP - 2064
JO - Journal of Economic Entomology
JF - Journal of Economic Entomology
SN - 0022-0493
IS - 4
ER -