Mouse brain fixation to preserve in vivo manganese enhancement for ex vivo manganese-enhanced MRI

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Purpose To develop a tissue fixation method that preserves in vivo manganese enhancement for ex vivo magnetic resonance imaging (MRI). The needs are clear, as conventional in vivo manganese-enhanced MRI (MEMRI) applied to live animals is time-limited, hence limited in spatial resolution and signal-to-noise ratio (SNR). Ex vivo applications can achieve superior spatial resolution and SNR through increased signal averaging and optimized radiofrequency coil designs. A tissue fixation method that preserves in vivo Mn2+ enhancement postmortem is necessary for ex vivo MEMRI. Materials and Methods T1 measurements and T1-weighted MRI were performed on MnCl2-administered mice. The mice were then euthanized and the brains were fixed using one of two brain tissue fixation methods: aldehyde solution or focused beam microwave irradiation (FBMI). MRI was then performed on the fixed brains. Results T1 values and T 1-weighted signal contrasts were comparable between in vivo and ex vivo scans on aldehyde-fixed brains. FBMI resulted in the loss of Mn 2+ enhancement. Conclusion Aldehyde fixation, not FBMI, maintained in vivo manganese enhancement for ex vivo MEMRI. © 2013 Wiley Periodicals, Inc.

Original languageEnglish (US)
Pages (from-to)482-487
Number of pages6
JournalJournal of Magnetic Resonance Imaging
Volume38
Issue number2
DOIs
StatePublished - Aug 2013

Keywords

  • MRI
  • aldehyde
  • ex vivo MRI
  • focused beam microwave irradiation
  • manganese enhanced MRI
  • tissue fixation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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