Mouse genome editing using the CRISPR/Cas system

Donald W. Harms, Rolen M. Quadros, Davide Seruggia, Masato Ohtsuka, Gou Takahashi, Lluis Montoliu, Channabasavaiah B. Gurumurthy

Research output: Contribution to journalArticlepeer-review

108 Scopus citations


The availability of techniques to create desired genetic mutations has enabled the laboratory mouse as an extensively used model organism in biomedical research including human genetics. A new addition to this existing technical repertoire is the CRISPR/Cas system. Specifically, this system allows editing of the mouse genome much more quickly than the previously used techniques, and, more importantly, multiple mutations can be created in a single experiment. Here we provide protocols for preparation of CRISPR/Cas reagents and microinjection into one-cell mouse embryos to create knockout or knock-in mouse models.

Original languageEnglish (US)
Pages (from-to)15.7.1-15.7.27
JournalCurrent Protocols in Human Genetics
StatePublished - 2014


  • CRISPR/Cas9
  • Gene editing
  • Mutant mouse
  • Pronuclear and cytoplasmic injection
  • sgRNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)


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