Mouse genome editing using the CRISPR/Cas system

Donald W. Harms, Rolen M. Quadros, Davide Seruggia, Masato Ohtsuka, Gou Takahashi, Lluis Montoliu, Channabasavaiah B. Gurumurthy

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

The availability of techniques to create desired genetic mutations has enabled the laboratory mouse as an extensively used model organism in biomedical research including human genetics. A new addition to this existing technical repertoire is the CRISPR/Cas system. Specifically, this system allows editing of the mouse genome much more quickly than the previously used techniques, and, more importantly, multiple mutations can be created in a single experiment. Here we provide protocols for preparation of CRISPR/Cas reagents and microinjection into one-cell mouse embryos to create knockout or knock-in mouse models.

Original languageEnglish (US)
Pages (from-to)15.7.1-15.7.27
JournalCurrent Protocols in Human Genetics
Volume83
DOIs
StatePublished - Jan 1 2014

Keywords

  • CRISPR/Cas9
  • Gene editing
  • Mutant mouse
  • Pronuclear and cytoplasmic injection
  • sgRNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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  • Cite this

    Harms, D. W., Quadros, R. M., Seruggia, D., Ohtsuka, M., Takahashi, G., Montoliu, L., & Gurumurthy, C. B. (2014). Mouse genome editing using the CRISPR/Cas system. Current Protocols in Human Genetics, 83, 15.7.1-15.7.27. https://doi.org/10.1002/0471142905.hg1507s83