Multi-functional magnetic nanoparticles for magnetic resonance imaging and cancer therapy

Murali M. Yallapu; Shadi F. Othman; Evan T. Curtis; Brij K. Gupta; Meena Jaggi; Subhash C. Chauhan

doi:10.1016/j.biomaterials.2010.11.028

Multi-functional magnetic nanoparticles for magnetic resonance imaging and cancer therapy

Murali M. Yallapu, Shadi F. Othman, Evan T. Curtis, Brij K. Gupta, Meena Jaggi, Subhash C. Chauhan

Research output: Contribution to journal › Article › peer-review

435 Scopus citations

Abstract

We have developed a multi-layer approach for the synthesis of water-dispersible superparamagnetic iron oxide nanoparticles for hyperthermia, magnetic resonance imaging (MRI) and drug delivery applications. In this approach, iron oxide core nanoparticles were obtained by precipitation of iron salts in the presence of ammonia and provided β-cyclodextrin and pluronic polymer (F127) coatings. This formulation (F127250) was highly water dispersible which allowed encapsulation of the anti-cancer drug(s) in β-cyclodextrin and pluronic polymer for sustained drug release. The F127250 formulation has exhibited superior hyperthermia effects over time under alternating magnetic field compared to pure magnetic nanoparticles (MNP) and β-cyclodextrin coated nanoparticles (CD200). Additionally, the improved MRI characteristics were also observed for the F127250 formulation in agar gel and in cisplatin resistant ovarian cancer cells (A12780CP) compared to MNP and CD200 formulations. Furthermore, the drug-loaded formulation of F127250 exhibited many folds of imaging contrast properties. Due to the internalization capacity of the F127250 formulation, its curcumin-loaded formulation (F127250-CUR) exhibited almost equivalent inhibition effects on A2780CP (ovarian), MDA-MB-231 (breast), and PC-3 (prostate) cancer cells even though curcumin release was only 40%. The improved therapeutic effects were verified by examining molecular effects using Western blotting and transmission electron microscopic (TEM) studies. F127250-CUR also exhibited haemocompatibility, suggesting a nanochemo-therapuetic agent for cancer therapy.

Original language	English (US)
Pages (from-to)	1890-1905
Number of pages	16
Journal	Biomaterials
Volume	32
Issue number	7
DOIs	https://doi.org/10.1016/j.biomaterials.2010.11.028
State	Published - Mar 2011
Externally published	Yes

Keywords

Drug delivery
Hyperthermia
MRI
Magnetic nanoparticles
Multi-layer coating

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

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10.1016/j.biomaterials.2010.11.028

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@article{bfdc7439eba544c596917282bf4b38d1,

title = "Multi-functional magnetic nanoparticles for magnetic resonance imaging and cancer therapy",

abstract = "We have developed a multi-layer approach for the synthesis of water-dispersible superparamagnetic iron oxide nanoparticles for hyperthermia, magnetic resonance imaging (MRI) and drug delivery applications. In this approach, iron oxide core nanoparticles were obtained by precipitation of iron salts in the presence of ammonia and provided β-cyclodextrin and pluronic polymer (F127) coatings. This formulation (F127250) was highly water dispersible which allowed encapsulation of the anti-cancer drug(s) in β-cyclodextrin and pluronic polymer for sustained drug release. The F127250 formulation has exhibited superior hyperthermia effects over time under alternating magnetic field compared to pure magnetic nanoparticles (MNP) and β-cyclodextrin coated nanoparticles (CD200). Additionally, the improved MRI characteristics were also observed for the F127250 formulation in agar gel and in cisplatin resistant ovarian cancer cells (A12780CP) compared to MNP and CD200 formulations. Furthermore, the drug-loaded formulation of F127250 exhibited many folds of imaging contrast properties. Due to the internalization capacity of the F127250 formulation, its curcumin-loaded formulation (F127250-CUR) exhibited almost equivalent inhibition effects on A2780CP (ovarian), MDA-MB-231 (breast), and PC-3 (prostate) cancer cells even though curcumin release was only 40%. The improved therapeutic effects were verified by examining molecular effects using Western blotting and transmission electron microscopic (TEM) studies. F127250-CUR also exhibited haemocompatibility, suggesting a nanochemo-therapuetic agent for cancer therapy.",

keywords = "Drug delivery, Hyperthermia, MRI, Magnetic nanoparticles, Multi-layer coating",

author = "Yallapu, {Murali M.} and Othman, {Shadi F.} and Curtis, {Evan T.} and Gupta, {Brij K.} and Meena Jaggi and Chauhan, {Subhash C.}",

note = "Funding Information: We thank Cathy Christopherson (Sanford Research/USD, Sioux Falls) for editorial assistance, Robert Japs (Sanford Health), Sara Basiaga (Department of Chemistry, UN-Lincoln), Shah R Valloppilly (Nebraska Center for Materials and Nanoscience, Department of Physics and Astronomy, UN-Lincoln), and Crittenden J. Ohlemacher (Applied Polymer Research Center, University of Akron) for their help in characterization of our samples. We also thank Dr. Omathanu P. Perumal (Department of Pharmaceutical Sciences, South Dakota State University, Brookings, SD) for providing access to DLS instrument for particles size and zeta potential measurements. The authors also thank Dr. Diane Maher, Dr. Vasudha Sundram and Mara Ebeling (Sanford Research/USD) for their suggestions throughout this study. This work was supported by grants from Sanford Research/USD , Department of Defense (DOD) ( PC073887 ), Governor{\textquoteright}s Cancer 2010 , and NIH RO1 ( CA142736 ) awarded to SCC and Department of Defense (DOD) ( PC073643 ) and Governor{\textquoteright}s Cancer 2010 grants awarded to MJ. ",

year = "2011",

month = mar,

doi = "10.1016/j.biomaterials.2010.11.028",

language = "English (US)",

volume = "32",

pages = "1890--1905",

journal = "Biomaterials",

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AU - Othman, Shadi F.

AU - Curtis, Evan T.

AU - Gupta, Brij K.

AU - Jaggi, Meena

AU - Chauhan, Subhash C.

N1 - Funding Information: We thank Cathy Christopherson (Sanford Research/USD, Sioux Falls) for editorial assistance, Robert Japs (Sanford Health), Sara Basiaga (Department of Chemistry, UN-Lincoln), Shah R Valloppilly (Nebraska Center for Materials and Nanoscience, Department of Physics and Astronomy, UN-Lincoln), and Crittenden J. Ohlemacher (Applied Polymer Research Center, University of Akron) for their help in characterization of our samples. We also thank Dr. Omathanu P. Perumal (Department of Pharmaceutical Sciences, South Dakota State University, Brookings, SD) for providing access to DLS instrument for particles size and zeta potential measurements. The authors also thank Dr. Diane Maher, Dr. Vasudha Sundram and Mara Ebeling (Sanford Research/USD) for their suggestions throughout this study. This work was supported by grants from Sanford Research/USD , Department of Defense (DOD) ( PC073887 ), Governor’s Cancer 2010 , and NIH RO1 ( CA142736 ) awarded to SCC and Department of Defense (DOD) ( PC073643 ) and Governor’s Cancer 2010 grants awarded to MJ.

PY - 2011/3

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N2 - We have developed a multi-layer approach for the synthesis of water-dispersible superparamagnetic iron oxide nanoparticles for hyperthermia, magnetic resonance imaging (MRI) and drug delivery applications. In this approach, iron oxide core nanoparticles were obtained by precipitation of iron salts in the presence of ammonia and provided β-cyclodextrin and pluronic polymer (F127) coatings. This formulation (F127250) was highly water dispersible which allowed encapsulation of the anti-cancer drug(s) in β-cyclodextrin and pluronic polymer for sustained drug release. The F127250 formulation has exhibited superior hyperthermia effects over time under alternating magnetic field compared to pure magnetic nanoparticles (MNP) and β-cyclodextrin coated nanoparticles (CD200). Additionally, the improved MRI characteristics were also observed for the F127250 formulation in agar gel and in cisplatin resistant ovarian cancer cells (A12780CP) compared to MNP and CD200 formulations. Furthermore, the drug-loaded formulation of F127250 exhibited many folds of imaging contrast properties. Due to the internalization capacity of the F127250 formulation, its curcumin-loaded formulation (F127250-CUR) exhibited almost equivalent inhibition effects on A2780CP (ovarian), MDA-MB-231 (breast), and PC-3 (prostate) cancer cells even though curcumin release was only 40%. The improved therapeutic effects were verified by examining molecular effects using Western blotting and transmission electron microscopic (TEM) studies. F127250-CUR also exhibited haemocompatibility, suggesting a nanochemo-therapuetic agent for cancer therapy.

AB - We have developed a multi-layer approach for the synthesis of water-dispersible superparamagnetic iron oxide nanoparticles for hyperthermia, magnetic resonance imaging (MRI) and drug delivery applications. In this approach, iron oxide core nanoparticles were obtained by precipitation of iron salts in the presence of ammonia and provided β-cyclodextrin and pluronic polymer (F127) coatings. This formulation (F127250) was highly water dispersible which allowed encapsulation of the anti-cancer drug(s) in β-cyclodextrin and pluronic polymer for sustained drug release. The F127250 formulation has exhibited superior hyperthermia effects over time under alternating magnetic field compared to pure magnetic nanoparticles (MNP) and β-cyclodextrin coated nanoparticles (CD200). Additionally, the improved MRI characteristics were also observed for the F127250 formulation in agar gel and in cisplatin resistant ovarian cancer cells (A12780CP) compared to MNP and CD200 formulations. Furthermore, the drug-loaded formulation of F127250 exhibited many folds of imaging contrast properties. Due to the internalization capacity of the F127250 formulation, its curcumin-loaded formulation (F127250-CUR) exhibited almost equivalent inhibition effects on A2780CP (ovarian), MDA-MB-231 (breast), and PC-3 (prostate) cancer cells even though curcumin release was only 40%. The improved therapeutic effects were verified by examining molecular effects using Western blotting and transmission electron microscopic (TEM) studies. F127250-CUR also exhibited haemocompatibility, suggesting a nanochemo-therapuetic agent for cancer therapy.

KW - Drug delivery

KW - Hyperthermia

KW - MRI

KW - Magnetic nanoparticles

KW - Multi-layer coating

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JF - Biomaterials

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ER -

Multi-functional magnetic nanoparticles for magnetic resonance imaging and cancer therapy

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Keywords

ASJC Scopus subject areas

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