Abstract
Mn2+-stimulated adenylate cyclase (ATP pyrophosphate-lyase-(cyclizing), EC 4.6.1.1) activity in detergent solubilized preparations from mouse brain. While NaF-stimulated activity was decreased by both solubilization and storage at 0-4°C, the ability of the enzyme to be stimulated by Mn2+ was maintained for up to one week. By including Mn+ in the assay of adenylate cyclase in gel fractions after isoelectric focusing, two distinct peaks of enzyme activity (pI1 = 5.8, pI2 = 6.4) were detected, suggesting the existence of more than one type of catalytic subunit in mouse brain cell membranes.
Original language | English (US) |
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Pages (from-to) | 243-247 |
Number of pages | 5 |
Journal | BBA - Enzymology |
Volume | 485 |
Issue number | 1 |
DOIs | |
State | Published - Nov 23 1977 |
Externally published | Yes |
ASJC Scopus subject areas
- Medicine(all)