Abstract
Mn2+-stimulated adenylate cyclase (ATP pyrophosphate-lyase-(cyclizing), EC 4.6.1.1) activity in detergent solubilized preparations from mouse brain. While NaF-stimulated activity was decreased by both solubilization and storage at 0-4°C, the ability of the enzyme to be stimulated by Mn2+ was maintained for up to one week. By including Mn+ in the assay of adenylate cyclase in gel fractions after isoelectric focusing, two distinct peaks of enzyme activity (pI1 = 5.8, pI2 = 6.4) were detected, suggesting the existence of more than one type of catalytic subunit in mouse brain cell membranes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 243-247 |
| Number of pages | 5 |
| Journal | BBA - Enzymology |
| Volume | 485 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 23 1977 |
ASJC Scopus subject areas
- Medicine(all)
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Malamuda, D. F., DiRusso, C. C., & Aprille, J. R. (1977). Multiple forms of brain adenylate cyclase: Stimulation by Mn2+. BBA - Enzymology, 485(1), 243-247. https://doi.org/10.1016/0005-2744(77)90211-X