Multiple forms of brain adenylate cyclase: Stimulation by Mn2+

Daniel F. Malamuda, Concetta C. DiRusso, June R. Aprille

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Mn2+-stimulated adenylate cyclase (ATP pyrophosphate-lyase-(cyclizing), EC activity in detergent solubilized preparations from mouse brain. While NaF-stimulated activity was decreased by both solubilization and storage at 0-4°C, the ability of the enzyme to be stimulated by Mn2+ was maintained for up to one week. By including Mn+ in the assay of adenylate cyclase in gel fractions after isoelectric focusing, two distinct peaks of enzyme activity (pI1 = 5.8, pI2 = 6.4) were detected, suggesting the existence of more than one type of catalytic subunit in mouse brain cell membranes.

Original languageEnglish (US)
Pages (from-to)243-247
Number of pages5
JournalBBA - Enzymology
Issue number1
StatePublished - Nov 23 1977
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine


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