Multiplex PCR−based next-generation sequencing and global diversity of seoul virus in humans and rats

Won Keun Kim, Jin Sun No, Seung Ho Lee, Dong Hyun Song, Daesang Lee, Jeong Ah Kim, Se Hun Gu, Sunhye Park, Seong Tae Jeong, Heung Chul Kim, Terry A. Klein, Michael R. Wiley, Gustavo Palacios, Jin Won Song

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Seoul virus (SEOV) poses a worldwide public health threat. This virus, which is harbored by Rattus norvegicus and R. rattus rats, is the causative agent of hemorrhagic fever with renal syndrome (HFRS) in humans, which has been reported in Asia, Europe, the Americas, and Africa. Defining SEOV genome sequences plays a critical role in development of preventive and therapeutic strategies against the unique worldwide hantavirus. We applied multiplex PCR-based next-generation sequencing to obtain SEOV genome sequences from clinical and reservoir host specimens. Epidemiologic surveillance of R. norvegicus rats in South Korea during 2000-2016 demonstrated that the serologic prevalence of enzootic SEOV infections was not significant on the basis of sex, weight (age), and season. Viral loads of SEOV in rats showed wide dissemination in tissues and dynamic circulation among populations. Phylogenetic analyses showed the global diversity of SEOV and possible genomic configuration of genetic exchanges.

Original languageEnglish (US)
Pages (from-to)249-257
Number of pages9
JournalEmerging infectious diseases
Issue number2
StatePublished - Feb 2018
Externally publishedYes

ASJC Scopus subject areas

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases


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