TY - JOUR
T1 - Mutations in a conserved enteroviral RNA oligonucleotide sequence affect positive strand viral RNA synthesis
AU - Willian, S.
AU - Tracy, S.
AU - Chapman, N.
AU - Leser, S.
AU - Romero, J.
AU - Shapiro, B.
AU - Currey, K.
PY - 2000
Y1 - 2000
N2 - We showed earlier that a transition mutation U234C, located within the completely conserved 5 nucleotide (nt) tract 5'-CGUUA (nt232-236) in the 5' non-translated region (NTR) of the coxsackievirus B3 (CVB3) genome, attenuated CVB3 cardiovirulence in mice. To further explore the role of the sequence, we induced two single and one double transversion mutations in the conserved 5mer in a cardiovirulent CVB3 genome. The mutated sites partially or totally reverted to parental wild-type when progeny viruses were passaged at 37 °C, but remained stable when transfection and subsequent passages were performed at 33.5 °C. Viral replication in cell culture was attenuated at 37 °C or 39.5 °C relative to replication at 33.5 °C. While Western blot analysis demonstrated the level of protein translation consistent with virus replication, the ratios of positive to negative strand viral RNA at 37 °C in murine cells demonstrated a 2-5 fold diminution from those measured at 33.5 °C. Mutant CVB3 strains failed to replicate productively when inoculated into mice. The biological data are consistent with an hypothesis that proposes a lesion with primary effects at the level of positive strand viral RNA synthesis that results in attenuation of viral replication at physiologic temperature.
AB - We showed earlier that a transition mutation U234C, located within the completely conserved 5 nucleotide (nt) tract 5'-CGUUA (nt232-236) in the 5' non-translated region (NTR) of the coxsackievirus B3 (CVB3) genome, attenuated CVB3 cardiovirulence in mice. To further explore the role of the sequence, we induced two single and one double transversion mutations in the conserved 5mer in a cardiovirulent CVB3 genome. The mutated sites partially or totally reverted to parental wild-type when progeny viruses were passaged at 37 °C, but remained stable when transfection and subsequent passages were performed at 33.5 °C. Viral replication in cell culture was attenuated at 37 °C or 39.5 °C relative to replication at 33.5 °C. While Western blot analysis demonstrated the level of protein translation consistent with virus replication, the ratios of positive to negative strand viral RNA at 37 °C in murine cells demonstrated a 2-5 fold diminution from those measured at 33.5 °C. Mutant CVB3 strains failed to replicate productively when inoculated into mice. The biological data are consistent with an hypothesis that proposes a lesion with primary effects at the level of positive strand viral RNA synthesis that results in attenuation of viral replication at physiologic temperature.
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U2 - 10.1007/s007050070040
DO - 10.1007/s007050070040
M3 - Article
C2 - 11087092
AN - SCOPUS:0033743884
SN - 0304-8608
VL - 145
SP - 2061
EP - 2086
JO - Archives of Virology
JF - Archives of Virology
IS - 10
ER -