Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7

Gaylen A. Uhlich; James E. Keen; Robert O. Elder

doi:10.1128/AEM.67.5.2367-2370.2001

Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7

Gaylen A. Uhlich, James E. Keen, Robert O. Elder

Research output: Contribution to journal › Article › peer-review

105 Scopus citations

Abstract

Single-base-pair csgD promoter mutations in human outbreak Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 coincided with differential Congo red dye binding from curli fiber expression. Red phenotype csgD::lacZ promoter fusions had fourfold-greater expression than white promoter fusions. Cloning the red variant csgDEFG operon into white variants induced the red phenotype. Substrate utilization differed between red and white variants.

Original language	English (US)
Pages (from-to)	2367-2370
Number of pages	4
Journal	Applied and environmental microbiology
Volume	67
Issue number	5
DOIs	https://doi.org/10.1128/AEM.67.5.2367-2370.2001
State	Published - May 2001
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

Access to Document

10.1128/AEM.67.5.2367-2370.2001

Cite this

@article{a2d6c433140a4802849f34f0ac68c31a,

title = "Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7",

abstract = "Single-base-pair csgD promoter mutations in human outbreak Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 coincided with differential Congo red dye binding from curli fiber expression. Red phenotype csgD::lacZ promoter fusions had fourfold-greater expression than white promoter fusions. Cloning the red variant csgDEFG operon into white variants induced the red phenotype. Substrate utilization differed between red and white variants.",

author = "Uhlich, {Gaylen A.} and Keen, {James E.} and Elder, {Robert O.}",

year = "2001",

month = may,

doi = "10.1128/AEM.67.5.2367-2370.2001",

language = "English (US)",

volume = "67",

pages = "2367--2370",

journal = "Applied and environmental microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "5",

}

TY - JOUR

T1 - Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7

AU - Uhlich, Gaylen A.

AU - Keen, James E.

AU - Elder, Robert O.

PY - 2001/5

Y1 - 2001/5

N2 - Single-base-pair csgD promoter mutations in human outbreak Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 coincided with differential Congo red dye binding from curli fiber expression. Red phenotype csgD::lacZ promoter fusions had fourfold-greater expression than white promoter fusions. Cloning the red variant csgDEFG operon into white variants induced the red phenotype. Substrate utilization differed between red and white variants.

AB - Single-base-pair csgD promoter mutations in human outbreak Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 coincided with differential Congo red dye binding from curli fiber expression. Red phenotype csgD::lacZ promoter fusions had fourfold-greater expression than white promoter fusions. Cloning the red variant csgDEFG operon into white variants induced the red phenotype. Substrate utilization differed between red and white variants.

UR - http://www.scopus.com/inward/record.url?scp=0035348261&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035348261&partnerID=8YFLogxK

U2 - 10.1128/AEM.67.5.2367-2370.2001

DO - 10.1128/AEM.67.5.2367-2370.2001

M3 - Article

C2 - 11319125

AN - SCOPUS:0035348261

SN - 0099-2240

VL - 67

SP - 2367

EP - 2370

JO - Applied and environmental microbiology

JF - Applied and environmental microbiology

IS - 5

ER -

Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this