Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7

Gaylen A. Uhlich, James E. Keen, Robert O. Elder

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

Single-base-pair csgD promoter mutations in human outbreak Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 coincided with differential Congo red dye binding from curli fiber expression. Red phenotype csgD::lacZ promoter fusions had fourfold-greater expression than white promoter fusions. Cloning the red variant csgDEFG operon into white variants induced the red phenotype. Substrate utilization differed between red and white variants.

Original languageEnglish (US)
Pages (from-to)2367-2370
Number of pages4
JournalApplied and environmental microbiology
Volume67
Issue number5
DOIs
StatePublished - May 2001

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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