Myeloblast phenotypic changes in myelodysplasia: CD34 and CD117 expression abnormalities are common

Samuel J. Pirruccello, Ken H. Young, Patricia Aoun

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

We used a new method of data presentation and analysis, termed antigen mapping, to characterize recurring myeloblast phenotypic abnormalities in a series of 28 cases of myelodysplastic syndrome (MDS), including refractory anemia with ringed sideroblasts (RARS), refractory anemia with multilineage dysplasia (RCMLD), and refractory anemia with excess blasts (RAEB). Abnormal patterns of CD34 and CD117 expression were present in 50% of RARS, 68% of RCMLD, and 100% of RAEB cases. The presence of decreased myeloblast CD45 density, increased CD13 and CD34 density, and increased expression of CD11c and CD4 dim were MDS grade-related. There was a direct relationship between the number of myeloblast phenotypic abnormalities (phenotypic score) and MDS grade. The myeloblast phenotypic scores also were correlated highly with International Prognostic Scoring System scores and risk categories. We found the antigen mapping technique to be an efficient data presentation and analysis method for the detection of MDS-associated abnormalities of antigen distribution and density.

Original languageEnglish (US)
Pages (from-to)884-894
Number of pages11
JournalAmerican journal of clinical pathology
Volume125
Issue number6
DOIs
StatePublished - Jun 2006

Keywords

  • Antigen density
  • Antigen mapping
  • CD117
  • CD34
  • Flow cytometry
  • International Prognostic Scoring System
  • Myelodysplastic syndrome
  • Phenotypic score

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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