N-glycoprotein surfaceome of human induced pluripotent stem cell derived hepatic endoderm

Sunil K. Mallanna, Matthew Waas, Stephen A. Duncan, Rebekah L. Gundry

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Using cell surface capture technology, the cell surface N-glycoproteome of human-induced pluripotent stem cell derived hepatic endoderm cells was assessed. Altogether, 395 cell surface N-glycoproteins were identified, represented by 1273 N-glycopeptides. This study identified N-glycoproteins that are not predicted to be localized to the cell surface and provides experimental data that assist in resolving ambiguous or incorrectly annotated transmembrane topology annotations. In a proof-of-concept analysis, combining these data with other cell surface proteome datasets is useful for identifying potentially cell type and lineage restricted markers and drug targets to advance the use of stem cell technologies for mechanistic developmental studies, disease modeling, drug discovery, and regenerative medicine.

Original languageEnglish (US)
Article number1600397
JournalProteomics
Volume17
Issue number5
DOIs
StatePublished - Mar 1 2017

Keywords

  • Cell surface proteins
  • Glycoproteomics
  • Hepatic endoderm
  • N-glycoproteins
  • Plasma membrane

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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