N-glycoprotein surfaceomes of four developmentally distinct mouse cell types

Erin M. Kropp, Subarna Bhattacharya, Matthew Waas, Sandra L. Chuppa, Anna Katerina Hadjantonakis, Kenneth R. Boheler, Rebekah L. Gundry

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Purpose: Detailed knowledge of cell surface proteins present during early embryonic development remains limited for most cell lineages. Due to the relevance of cell surface proteins in their functional roles controlling cell signaling and their utility as accessible, nongenetic markers for cell identification and sorting, the goal of this study was to provide new information regarding the cell surface proteins present during early mouse embryonic development. Experimental Design: Using the cell surface capture technology, the cell surface N-glycoproteomes of three cell lines and one in vitro differentiated cell type representing distinct cell fates and stages in mouse embryogenesis were assessed. Results: Altogether, more than 600 cell surface N-glycoproteins were identified represented by >5500 N-glycopeptides. Conclusions and Clinical Relevance: The development of new, informative cell surface markers for the reliable identification and isolation of functionally defined subsets of cells from early developmental stages will advance the use of stem cell technologies for mechanistic developmental studies, including disease modeling and drug discovery.

Original languageEnglish (US)
Pages (from-to)603-609
Number of pages7
JournalProteomics - Clinical Applications
Volume8
Issue number7-8
DOIs
StatePublished - Aug 2014

Keywords

  • Biomarkers
  • Cardiovascular
  • Glycoproteins
  • Glycoproteomics
  • Plasma membrane

ASJC Scopus subject areas

  • Clinical Biochemistry

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    Kropp, E. M., Bhattacharya, S., Waas, M., Chuppa, S. L., Hadjantonakis, A. K., Boheler, K. R., & Gundry, R. L. (2014). N-glycoprotein surfaceomes of four developmentally distinct mouse cell types. Proteomics - Clinical Applications, 8(7-8), 603-609. https://doi.org/10.1002/prca.201400021